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(E,E)-3,5-Bis(phenylethenyl)-BDP is a symmetric one of bis(hetero)arylethenyl-substituted boron difluoride dipyrromethene derivatives. The molecule has two phenylethenyl groups in trans configuration of the double bonds, which positively impact to lipophilicity. This non-functionalized derivative of the BDP dye is expected to accumulate in lipid droplets (LDs). BDP dyes exhibit high brightness, and excellent photostability, and can be used as fluorogenic probes for imaging of LDs.
An aromatic, alkenyl or alkynyl substitution is considered to extend the π-conjugation by introducing at the 3,5-positions of the core and thus the red-shifted fluorescence emission wavelength compared to most lipid droplet markers from the classical BDP dye series is observed.
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