BO-TAP-3, orange fluorescent nucleic acid stain

Cat. # Quantity Price Lead time
A4910 50 uL, 5 mM/DMSO $85 in stock
24910 200 uL, 5 mM/DMSO $319 in stock
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BO-TAP-3 (also known as BO-PRO®-3) is a orange fluorescent carbocyanine monomeric dye. BO-TAP-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make BO-TAP-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.

The dye is used to image cell nuclei and cytoplasm, label bacteria and sperms, perform nucleic acid detection and amplification, and as a temperature sensor.

Excitation and emission spectra of BO-TAP-3 (DNA–dye complex)

Excitation and emission spectra of BO-TAP-3 (DNA–dye complex)

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General properties

Appearance: purple solution
Molecular weight: 621.37
CAS number: 173357-16-9
Molecular formula: C22H29I2N3S
IUPAC name: 3-methyl-2-((e)-3-[1-[3-(trimethylammonio)propyl]-4(1h)-pyridinylidene]-1-propenyl)-1,3-benzothiazol-3-ium diiodide
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 564 (complex)
Emission maximum, nm: 600 (complex)
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