LUCS® 13, green fluorescent nucleic acid stain

Nuclei Cell viability and apoptosis Microscopy Flow cytometry
Cat. # Quantity Price Lead time
14010 50 uL, 5 mM/DMSO $110 in stock
24010 250 uL, 5 mM/DMSO $250 in stock
44010 1 mL, 5 mM/DMSO $750 in stock
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LUCS 13 is a cell-permeant nucleic acid stain that exhibits green fluorescence upon binding to nucleic acids. The stain has a high fluorescent yield and a structure identical to SYTO™13 stain.

LUCS 13 is used to stain both DNA and RNA in live and dead eukaryotic cells as well as Gram-positive and Gram-negative bacteria. The dye is excited by the blue laser at 488 nm. Its fluorescence emission is detected in the fluorescein channel with a peak at 509 nm when bound to DNA and 514 nm when bound to RNA.

The dye can be used in simultaneous labeling with cell-impermeant nuclear markers, such as YoDi-3, to evaluate cell viability using fluorescence microscopy and flow cytometry.

LUCS 13, green fluorescent nucleic acid stain, 24010
LUCS 13, green fluorescent nucleic acid stain, 24010
LUCS 13, green fluorescent nucleic acid stain, 24010
Human blood smear stained with green fluorescent nuclear stain LUCS13 and antibodies to neutrophil myeloperoxidase. Human blood smear stained with green fluorescent nuclear stain LUCS13 (x4010) and antibodies to neutrophil myeloperoxidase. Antibodies were labeled with red fluorescent dye sulfo-Cyanine5 using the Lumiprobe sulfo-Cyanine5 antibody labeling kit (3321-x).
Live RAW 264.7 cells stained with cell-penetrating nuclear dye LUCS 13 (0.5 µM, x4010, analog of SYTO™13) and PKH26 cell membrane labeling kit (x3201). Cells were counterstained with the nuclear dye Hoechst 33342.

Absorption and emission spectra of LUCS 13

Absorption and emission spectra of LUCS 13

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General properties

Appearance: orange solution
Solubility: miscible with water
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 488
Emission maximum, nm: 509 (DNA complex), 514 (RNA complex)

Product citations

  1. Suwatthanarak, T.; Tanjak, P.; Chaiboonchoe, A.; Acharayothin, O.; Thanormjit, K.; Chanthercrob, J.; Suwatthanarak, T.; Niyomchan, A.; Tanaka, M.; Okochi, M.; Pongpaibul, A.; Chalermwai, W.V.; Trakarnsanga, A.; Methasate, A.; Pithukpakorn, M.; Chinswangwatanakul, V. Overexpression of TSPAN8 in consensus molecular subtype 3 colorectal cancer. Experimental and molecular pathology, 2024, 137, 104911. doi: 10.1016/j.yexmp.2024.104911
SYTO® is a trademark of Molecular Probes Inc.
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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