ProteOrange® Protein Quantification Reagent, 500×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11210 | 100 uL | – | in stock | |
| 41210 | 1 mL | $195 | in stock |
ProteOrange® is a fluorescent protein-sensitive dye that exhibits almost no fluorescence in the free form but fluoresces upon binding to proteins. The excitation maximum of ProteOrange in the complex with protein is at ~485 nm, the emission maximum — at ~590 nm.
The unique structure of the ProteOrange reagent allows accurate detection of proteins in solutions with concentrations between 10 ng/mL and 10 µg/mL. This level of sensitivity is considerably better than that achieved with the BCA method, Bradford assay, Lowry assay, or absorption measurements at 280 nm. The dye shows minimal variability for different proteins.
The ProteOrange dye has a low tolerance to non-ionic detergents. It is not recommended for cell lysates containing Triton X-100 and sodium deoxycholate, but it can tolerate up to 0.01% SDS (in final concentration).
This reagent is a 500× concentrate of ProteOrange dye. This product is a component of ProteOrange Protein Quantification Kit.
Excitation and emission spectra of ProteOrange complex with protein
Customers also purchased with this product
Fluo-4 AM, green fluorescent calcium indicator
Fluo-4 AM is a cell-permeable calcium ion indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon calcium ion binding. Fluo-4 AM is well suited for fluorometric and imaging applications.AF 488 NHS ester
AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.LumiTracker® Mito TMRE
Tetramethylrhodamine ethyl ester (TMRE) is an orange-red fluorescent dye for staining active mitochondria and quantitate mitochondrial membrane potential in live cells.General properties
| Appearance: | Orange solution |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 470 |
| Emission maximum, nm: | 570 |
Product citations
- Kladova, O.A.; Tyugashev, T.E.; Yakimov, D.V.; Mikushina, E.S.; Novopashina, D.S.; Kuznetsov, N.A.; Kuznetsova, A.A. The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities. International Journal of Molecular Sciences, 2024, 25(8), 4182. doi: 10.3390/ijms25084182
- Grigorenko, V.G.; Krivitskaya, A.V.; Khrenova, M.G.; Rubtsova, M.Y..; Presnova, G.V.; Andreeva, I.P.; Serova, O.V.; Egorov, A.M. Saturation Mutagenesis and Molecular Modeling: The Impact of Methionine 182 Substitutions on the Stability of β-Lactamase TEM-1. International Journal of Molecular Sciences, 2024, 25(14), 7691. doi: 10.3390/ijms25147691
- Berditchevskii, G.M.; Vasina, L.V.; Ageev, S.V.; Meshcheriakov, A.A.; Galkin, M.A.; Ishmukhametov, R.R.; Nashchekin, A.V.; Kirilenko, D.A.; Petrov, A.V.; Martynova, S.D.; Semenov, K.N.; Sharoyko, V.V. A comprehensive study of biocompatibility of detonation nanodiamonds. Journal of Molecular Liquids, 2021, 332, 115763. doi: 10.1016/j.molliq.2021.115763
Your cart is empty
$ 
