LUCS® 13, green fluorescent nucleic acid stain
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14010 | 50 uL, 5 mM/DMSO | $110 | in stock | |
| 24010 | 250 uL, 5 mM/DMSO | $250 | in stock | |
| 44010 | 1 mL, 5 mM/DMSO |
$750
|
in stock |
LUCS 13 is a cell-permeant nucleic acid stain that exhibits green fluorescence upon binding to nucleic acids. The stain has a high fluorescent yield and a structure identical to SYTO™13 stain.
LUCS 13 is used to stain both DNA and RNA in live and dead eukaryotic cells as well as Gram-positive and Gram-negative bacteria. The dye is excited by the blue laser at 488 nm. Its fluorescence emission is detected in the fluorescein channel with a peak at 509 nm when bound to DNA and 514 nm when bound to RNA.
The dye can be used in simultaneous labeling with cell-impermeant nuclear markers, such as YoDi-3, to evaluate cell viability using fluorescence microscopy and flow cytometry.
Product in action
Live MDCK renal epithelial cells stained with LumiTracker Lyso Blue (x8201), PKH26 (x3201), and LUCS 13 (x4010) dyes
Human blood smear stained with green fluorescent nuclear stain LUCS13 and antibodies to neutrophil myeloperoxidase.
Live RAW 264.7 cells stained with LUCS 13 (x4010) and Hoechst 33342 (xG010) nuclear dyes and PKH26 cell membrane labeling kit (x3201)
Excitation and emission spectra of dsDNA complex with LUCS® 13
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| Appearance: | orange solution |
| Solubility: | miscible with water |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 488 |
| Emission maximum, nm: | 509 (DNA complex), 514 (RNA complex) |
Product citations
- Suwatthanarak, T.; Tanjak, P.; Chaiboonchoe, A.; Acharayothin, O.; Thanormjit, K.; Chanthercrob, J.; Suwatthanarak, T.; Niyomchan, A.; Tanaka, M.; Okochi, M.; Pongpaibul, A.; Chalermwai, W.V.; Trakarnsanga, A.; Methasate, A.; Pithukpakorn, M.; Chinswangwatanakul, V. Overexpression of TSPAN8 in consensus molecular subtype 3 colorectal cancer. Experimental and molecular pathology, 2024, 137, 104911. doi: 10.1016/j.yexmp.2024.104911
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