DryDrops® qPCR, Single Assay qPCR Lyophilized Beads

Cat. # Quantity Price Lead time
11534 10 rxn $23 21 days
21534 50 rxn $115 21 days
31534
/ pre-dispensed
8 rxn $20 21 days
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Single Assay qPCR Lyophilized Beads are a pre-formulated and pre-dispensed reaction mixture for carrying out polymerase chain reaction (PCR). Each lyophilized bead contains all the necessary components for PCR and qPCR with a volume of 25 μL:

  • hot-start Taq polymerase;
  • mixture of deoxynucleoside triphosphates (dNTPs);
  • optimized buffer for PCR (contains Mg2+ with a concentration of 3 mM in 1× reaction mixture);
  • protectors for lyophilization.

The composition of the reaction mixture is optimized to obtain ideal results in terms of processivity and specificity of amplification. Lyophilized beads provide excellent reproducibility between reactions by minimizing pipetting steps and reducing the likelihood of pipetting errors and sample contamination. Each batch of the Single Assay qPCR Lyophilized Beads undergoes functional testing to ensure batch-to-batch reproducibility.

The beads are suitable for real-time PCR detection, including quantitative analysis (with fluorescent probes or intercalating dye, e.g., Eva488), as well as for DNA amplification with subsequent detection of results by electrophoresis. The beads are also suitable for routine cloning tasks and other applications that require further handling of the PCR product after amplification.

Single Assay qPCR Lyophilized Beads are compatible with any type of amplifier and can be used for PCR in amplifiers with classic thermal blocks for PCR tubes and amplifiers with cartridges.

The lyophilized format allows storing the PCR mixture for up to 12 months at temperatures up to 4 °C.

We offer Single Assay qPCR Lyophilized Beads in vials of 10 pcs. (11534), vials of 50 pcs. (21534), and PCR-strips of 8 pcs. (31534).

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General properties

Appearance: white smooth spheres
Storage conditions: 12 months from delivery at temperature up to 4 °C protected from light. Transportation: Up to 3 weeks at temperature up to 4 °C. Protect from moisture.
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