R6G alkyne, 6-isomer

Cat. # Quantity Price Lead time
A52B0 1 mg $110 in stock
B52B0 5 mg $210 in stock
C52B0 10 mg $310 in stock
D52B0 25 mg $410 in stock
E52B0 50 mg $695 in stock
F52B0 100 mg $1190 in stock
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Rhodamine 6G (R6G) is a xanthene dye of rhodamine series. Compared to fluorescein, its emission is shifted to red. It is also much more photostable. The fluorophore has a high quantum yield.

This R6G product contains a terminal alkyne moiety for the conjugation with azides in the presence of copper (I) catalyst.

Absorption and emission spectra of 6-R6G

Absorption and emission spectra of 6-R6G

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ROX alkyne, 5-isomer

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JOE alkyne, 6-isomer

JOE dye alkyne derivative for copper-catalyzed click chemistry reaction with azides. JOE dye is widely used for nucleic acid labeling.

General properties

Appearance: dark colored solid
Mass spec M+ increment: 494.2
Molecular weight: 495.57
Molecular formula: C30H29N3O4
Solubility: good in DMF, DMSO, alcohols
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 518
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 542
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17

Product citations

  1. Guarin, M.; Faelens, R.; Giusti, A.; De Croze, N.; Léonard, M.; Cabooter, D.; Annaert, P.; de Witte, P.; Ny, A. Spatiotemporal Imaging and Pharmacokinetic of Fluorescent Compounds in Zebrafish Eleuthero-Embryos After Different Routes of Administration. Scientific Reports, 2021, 11(1), 12229. doi: 10.1038/s41598-021-91612-6.
  2. Guarin, M.; Ny, A.; De Croze, N.; Maes, J.; Léonard, M.; Annaert, P.; de Witte, P. A. M. Pharmacokinetics in Zebrafish Embryos (ZFE) Following Immersion and Intrayolk Administration: A Fluorescence-Based Analysis. Pharmaceuticals, 2021, 14(6), 576. doi: 10.3390/ph14060576
  3. Guarin, M.; Faelens, R.; Giusti, A.; De Croze, N.; Léonard, M.; Cabooter, D.; Annaert, P.; de Witte, P.; Ny, A. Spatiotemporal imaging and pharmacokinetics of fluorescent compounds in zebrafish eleuthero-embryos after different routes of administration. Scientific Reports, 2021, 11, 12229. doi: 10.1038/s41598-021-91612-6
  4. Gao, F.; Hunter, A.; Qu, S.; Hoffman, J.R.; Gao, P.; Phillip, W.A. Interfacial Junctions Control Electrolyte Transport Through Charge-Patterned Membranes. ACS Nano, 2019, 13(7), 7655–7664. doi: 10.1021/acsnano.9b00780
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