Cyanine5 alkyne
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
A30B0 | 1 mg | $125 | in stock | |
B30B0 | 5 mg | $260 | in stock | |
C30B0 | 10 mg | $325 | in stock | |
D30B0 | 25 mg | $510 | in stock | |
E30B0 | 50 mg | $895 | in stock | |
F30B0 | 100 mg | $1490 | in stock |
Cyanine5 is an analog of Cy5®, deeply colored and photostable fluorophore. Cyanine5 alkyne can be attached to various molecules via click chemistry reaction with azides.
This alkyne is non-water soluble, but it can be dissolved in DMF or DMSO before reaction and added to an aqueous reaction mixture. With our labeling protocol, the labeling reaction is very efficient, and high-yielding.
Various substrates bearing azides can be used for the labeling, including azido-labeled biomolecules, polymers, and solid surfaces.
Absorbance and emission spectra of Cyanine5
Recommended protocol
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Near-infrared (NIR) fluorescent dye for click chemistry labeling.sulfo-Cyanine5.5 NHS ester
Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.Cyanine5 carboxylic acid
Cyanine5 carboxylic acid, unactivated dye. An analog of Cy5®; free carboxylic acid.General properties
Appearance: | dark blue powder |
Molecular weight: | 556.18 |
CAS number: | 1223357-57-0 |
Molecular formula: | C35H42ClN3O |
Solubility: | good in dichloromethane, DMF, DMSO, alcohols, very poorly soluble in water (200 mg/L = 0.4 mM) |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 646 |
ε, L⋅mol−1⋅cm−1: | 250000 |
Emission maximum, nm: | 662 |
Fluorescence quantum yield: | 0.2 |
CF260: | 0.03 |
CF280: | 0.04 |
Product citations
- Peng, Y.-J.; Nanduri, J.; , Wang, N.; Kumar, G.K.; Bindokas, V.;,Paul, B.D.; Chen, X.; Fox, A.P.; Vignane, T.; Filipovic, M.R.; Prabhakar, N.R. Hypoxia sensing requires H2S-dependent persulfidation of olfactory receptor 78. Science Advances, 2023, 9(27), eadf302. doi: 10.1126/sciadv.adf3026
- Drekolia, M.-K.; Karantanou, C.; Wittig, I.; Li, Y.; Fuhrmann, D.C.; Brüne, B.; Katsouda, A.; Hu, J.; Papapetropoulos, A.; Bibli, S.-I. Loss of cardiac mitochondrial complex I persulfidation impairs NAD + homeostasis in aging. Redox Biology, 2023, 69, 103014. doi: 10.1016/j.redox.2023.103014
- Sokolova, V.; Ebel, J.-F.; Kollenda, S.; Klein, K.; Kruse, B.; Veltkamp, C.; Lange, C. M.; Westendorf, A. M.; Epple, M. Uptake of Functional Ultrasmall Gold Nanoparticles in 3D Gut Cell Models. Small, 2022, 18(31), 2201167. doi: 10.1002/smll.202201167
- Sargiacomo, C.; Stonehouse, S.; Moftakhar, Z.; Sotgia, F.; Lisanti, M.P. MitoTracker Deep Red (MTDR) Is a Metabolic Inhibitor for Targeting Mitochondria and Eradicating Cancer Stem Cells (CSCs), With Anti-Tumor and Anti-Metastatic Activity In Vivo. Frontiers in Oncology, 2021, 11, 678343. doi: 10.3389/fonc.2021.678343