SIMA-dT phosphoramidite, 6-isomer

Cat. # Quantity Price Lead time
2950-100mg 100 mg $180 in stock
2950-250mg 250 mg $390 in stock
2950-1g 1 g $1120 in stock
2950-5g 5 g $4520 in stock
2950-10g 10 g $8650 in stock
Found better price? Let us know and we will propose the way forward!

SIMA-dT phosphoramidite is used to introduce SIMA in the sequence during oligonucleotide synthesis, usually as a substitute for the native dT linkage.

SIMA is known to be much more stable than HEX in basic media thus deprotection in harsh conditions using ammonium hydroxide (up to 6-8 hours at 55 °C) is possible as well as AMA at room temperature or at 65 °C.

Recommendations for using the reagent:

Coupling: 6 minutes coupling time recommended.

Deprotection: standard method recommended, can be deprotected with AMA (1:1 mixture of concentrated aqueous ammonium hydroxide / 40% aqueous methylamine).

Absorption and emission spectra of SIMA

Absorption and emission spectra of SIMA

Customers also purchased with this product

SIMA phosphoramidite, 6-isomer

A phosphoramidite for the synthesis of SIMA-labeled oligonucleotides. SIMA is a more stable analog of HEX.
Add this product to your cart and
get free express delivery

FAM phosphoramidite, 6-isomer (hydroxyprolinol)

Non-nucleoside phosphoramidite reagent suitable for labeling of oligonucleotides with fluorescein.
Add this product to your cart and
get free express delivery

Phosphate CPG 500 v2

Modified CPG for 3'-phosphorylation of an oligonucleotide, pore size of 500 Å.

General properties

Appearance: white powder
Molecular weight: 1646.67
Molecular formula: C91H95Cl2N6O17P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 531
ε, L⋅mol−1⋅cm−1: 92300
Emission maximum, nm: 555
Fluorescence quantum yield: 0.63
CF260: 0.57
CF280: 0.18

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Deprotection conditions: identical to protected nucleobases
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.