SIMA-dT phosphoramidite, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2950-100mg | 100 mg | $180 | in stock | |
| 2950-250mg | 250 mg | $390 | in stock | |
| 2950-1g | 1 g |
$1120
|
in stock | |
| 2950-5g | 5 g |
$4520
|
in stock | |
| 2950-10g | 10 g |
$8650
|
in stock |
SIMA-dT phosphoramidite is used to introduce SIMA in the sequence during oligonucleotide synthesis, usually as a substitute for the native dT linkage.
SIMA is known to be much more stable than HEX in basic media thus deprotection in harsh conditions using ammonium hydroxide (up to 6-8 hours at 55 °C) is possible as well as AMA at room temperature or at 65 °C.
Recommendations for using the reagent:
Coupling: 6 minutes coupling time recommended.
Deprotection: standard method recommended, can be deprotected with AMA (1:1 mixture of concentrated aqueous ammonium hydroxide / 40% aqueous methylamine).
Absorption and emission spectra of SIMA
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| Appearance: | white powder |
| Molecular weight: | 1646.67 |
| Molecular formula: | C91H95Cl2N6O17P |
| Solubility: | Good solubility in acetonitrile and DCM |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 531 |
| ε, L⋅mol−1⋅cm−1: | 92300 |
| Emission maximum, nm: | 555 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.57 |
| CF280: | 0.18 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | identical to protected nucleobases |
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