sulfo-Cyanine5 streptavidin
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A33S0 | 100 ug | – | in stock | |
| 133S0 | 1 mg | $185 | in stock |
Streptavidin is a tetrameric biotin-binding protein derived from the bacterium Streptomyces avidinii. Streptavidin binds up to four biotin molecules with high affinity and selectivity via multiple hydrogen bonds and van der Waals interactions. Due to the lack of carbohydrate modifications and a near-neutral pI, streptavidin exhibits less nonspecific binding than another biotin-binding protein — avidin. Streptavidin also has high thermostability and resistance against extreme pH, denaturing agents, and enzymatic degradation, allowing using this protein under various experimental conditions.
Fluorescent conjugates of streptavidin are commonly used as a second-step reagent for specific detection of a variety of biotin-labeled biomolecules, such as proteins (antibodies, etc.), nucleic acids, lipids, and other molecules in indirect immunofluorescent staining, western blots, flow cytometry, microplate assays, and other detection techniques.
This streptavidin is a lyophilized conjugate with sulfo-Cyanine5, a hydrophilic far-red fluorophore with spectral characteristics similar to Cy5® (absorption max. at 646 nm, emission max. at 662 nm). Far-red fluorescent tags with excitation above 600 nm and emission further than 650 nm are valuable for imaging techniques because of the lower background autofluorescence at these wavelengths.
The recommended concentration range for use is 0.5-10 µg/mL. Avoid using biotin-containing solutions (some serums, RPMI 1640, etc.) as diluents.
Absorption and emission spectra of sulfo-Cyanine5
Recommended protocol
Calculator
Customers also purchased with this product
sulfo-Cyanine5 maleimide
Thiol reactive maleimide, water soluble cyanine dye for Cy5 channel.LumiTracker® Mito JC-1
The cationic carbocyanine dye that is widely used as an indicator of mitochondrial membrane potential to study apoptosis and monitor the health of mitochondria.Cyanine5 NHS ester
Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.General properties
| Appearance: | blue solid |
| Solubility: | good in water |
| Storage conditions: | Transportation: at room temperature for 1 week. Store at -20°С 9 months. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Your cart is empty
$ 
