FAM-11-dUTP, 6-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
2077-10nmol | 10 nmol | – | in stock | |
2077-50nmol | 50 nmol | $190 | in stock | |
2077-250nmol | 250 nmol | $325 | in stock | |
2077-500nmol | 500 nmol | $500 | in stock | |
2077-1umol | 1 umol | $910 | in stock |
6-FAM-11-2’-deoxyuridine-5’-triphosphate, trilithium salt, is a common agent for non-radioactive DNA labeling.
FAM (fluorescein) is a popular green-fluorescent dye with an emission maximum at 513 nm. This derivative is a pure FAM 6-isomer.
6-FAM-11-dUTP is a modified triphosphate that can be incorporated into DNA instead of dTTP by various enzymes, including Klenow fragment, Taq-Polymerase, DNA-Polymerase I, Phi 29 Polymerase, reverse transcriptase or terminal transferase.
In contrast to triphosphates with a dye directly linked to aminoallyl-dUTP, this triphosphate contains a linker of 11 atoms between the fluorophore and the nitrogenous base. This linker length prevents FAM-label from potential static quenching and increases the efficiency of nucleotide incorporation during DNA synthesis.
6-FAM-11-dUTP can be used to produce a labeled product during PCR and cDNA synthesis and for labeled DNA-probes synthesis by random primed labeling and Nick-translation. Synthesized fluorescence-labeled DNA probes can be used to detect specific sequences by Southern blot, Northern blot, in situ hybridization, or by microarray analysis.
Absorption and emission spectra of FAM
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Appearance: | yellow to orange solid |
Molecular weight: | 1013.49 |
Molecular formula: | C39H39N4Li3O21P3 |
IUPAC name: | ((2R,3S,5R)-5-(5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
Solubility: | soluble in water |
Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 492 |
ε, L⋅mol−1⋅cm−1: | 74000 |
Emission maximum, nm: | 517 |
Fluorescence quantum yield: | 0.93 |
CF260: | 0.22 |
CF280: | 0.17 |