ssGreen RNA Gel Staining Solution, 10,000×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A2510 | 50 uL | – | in stock | |
| 12510 | 500 uL | $385 | in stock |
ssGreen® is one of the most sensitive stains for post-electrophoresis staining of RNA and single-stranded DNA (ssDNA) in agarose or polyacrylamide gels. The fluorescence quantum yield of the ssGreen/RNA complex is more than 7× higher than that of the ethidium bromide/RNA complex. Although ssGreen is not selective for RNA staining, the dye exhibits about 1.5× greater quantum yield when bound to RNA than double-stranded DNA, which makes it unique among all nucleic acid dyes.
ssGreen staining is compatible with denaturing gels. On agarose/formaldehyde and polyacrylamide/urea gels, the sensitivity of ssGreen is slightly reduced but still superior to that of ethidium bromide. Staining agarose/formaldehyde gels with ssGreen does not interfere with transfer of RNA to filters or subsequent hybridization in Northern blot analysis as long as 0.1%–0.3% SDS is included in prehybridization and hybridization buffers.
Staining gels with ssGreen has fewer steps than those with ethidium bromide. Because the fluorescence of ssGreen/RNA complexes is not quenched by formaldehyde or urea, there is no need to wash these denaturants out of gels before staining. Also, ssGreen stain has a low intrinsic fluorescence, allowing gel viewing and photographing without preliminary removing unbound dye.
Excitation and emission spectra of RNA complex with ssGreen
Recommended protocol
Calculator
Customers also purchased with this product
Cyanine3 hydrazide
Cyanine3 hydrazide is a carbonyl-reactive dye for Cy3 channel.AF 488 azide
AF 488 azide is a fluorophore with emission in the green spectrum range. It reacts with alkynes in copper-catalyzed cycloaddition reactions.BDP® 630/650-X-NHS ester
BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays. This is an amine-reactive NHS ester derivative.General properties
| Appearance: | dark orange solution |
| Molecular weight: | 581.52 |
| CAS number: | 172827-25-7 |
| Molecular formula: | C28H28IN3OS |
| Quality control: | UV-Vis abs |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 483 (complex) |
| Emission maximum, nm: | 518 (complex) |
Your cart is empty
$ 
