ssGreen RNA Gel Staining Solution, 10,000×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A2510 | 50 uL | – | in stock | |
| 12510 | 500 uL | $385 | in stock |
ssGreen® is one of the most sensitive stains for post-electrophoresis staining of RNA and single-stranded DNA (ssDNA) in agarose or polyacrylamide gels. The fluorescence quantum yield of the ssGreen/RNA complex is more than 7× higher than that of the ethidium bromide/RNA complex. Although ssGreen is not selective for RNA staining, the dye exhibits about 1.5× greater quantum yield when bound to RNA than double-stranded DNA, which makes it unique among all nucleic acid dyes.
ssGreen staining is compatible with denaturing gels. On agarose/formaldehyde and polyacrylamide/urea gels, the sensitivity of ssGreen is slightly reduced but still superior to that of ethidium bromide. Staining agarose/formaldehyde gels with ssGreen does not interfere with transfer of RNA to filters or subsequent hybridization in Northern blot analysis as long as 0.1%–0.3% SDS is included in prehybridization and hybridization buffers.
Staining gels with ssGreen has fewer steps than those with ethidium bromide. Because the fluorescence of ssGreen/RNA complexes is not quenched by formaldehyde or urea, there is no need to wash these denaturants out of gels before staining. Also, ssGreen stain has a low intrinsic fluorescence, allowing gel viewing and photographing without preliminary removing unbound dye.
Excitation and emission spectra of RNA complex with ssGreen
Recommended protocol
Calculator
Customers also purchased with this product
dsGreen Nucleic Acid Gel Staining Solution, 10,000×
dsGreen for staining dsDNA in agarose and polyacrylamide gels.TODi-1, green fluorescent nucleic acid stain
TODi-1 is a cell-impermeant green fluorescent nucleic acid stain. TODi-1 is chemically equivalent to TOTO®-1.AF 488 tyramide
A tyramide derivative of AF 488, a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.General properties
| Appearance: | dark orange solution |
| Molecular weight: | 581.52 |
| CAS number: | 172827-25-7 |
| Molecular formula: | C28H28IN3OS |
| Quality control: | UV-Vis abs |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 483 (complex) |
| Emission maximum, nm: | 518 (complex) |
Your cart is empty
$ 
