SynaptoProbe® Green

Cat. # Quantity Price Lead time
3762-100ug 100 ug –   in stock
3762-1mg 1 mg $132 in stock
3762-5mg 5 mg $350 in stock
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SynaptoProbe® Green is an analog of FM®1-43, a widely used green fluorescent dye for studying synaptic activity in the synapses or neuromuscular junctions by staining synaptic vesicles.

This water-soluble and non-toxic to cells dye is nonfluorescent in an aqueous medium but becomes highly fluorescent after embedding into the outer leaflet of the cell membrane. When a neuron actively releases neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and stains the nerve terminals.

SynaptoProbe® Green can be used to visualize synaptic vesicles and their exocytosis and endocytosis in living neurons, identify actively firing neurons, and investigate the mechanisms of activity-dependent vesicle cycling. It is also helpful for the visualization of shear stress-induced plasma membrane damage in fibroblasts.

Absorption and emission spectra of SynaptoProbe Green

Absorption and emission spectra of SynaptoProbe Green

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General properties

Appearance: light red solid
Molecular weight: 705.55
CAS number: 149838-22-2 (dibromide)
Molecular formula: C30H49I2N3
IUPAC name: N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Diiodide
Solubility: good in water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 55800
Emission maximum, nm: 594
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