Cyanine5 azide
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
13030 | 100 uL, 10 mM/DMSO | – | in stock | |
33030 | 500 uL, 10 mM/DMSO | $199 | in stock | |
43030 | 1 mL, 10 mM/DMSO | $495 | in stock | |
A3030 | 1 mg | – | in stock | |
B3030 | 5 mg | $199 | in stock | |
C3030 | 10 mg | $297 | in stock | |
D3030 | 25 mg | $495 | in stock | |
E3030 | 50 mg | $895 | in stock | |
F3030 | 100 mg | $1490 | in stock |
Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO and in solid form.
This azide is soluble in organic solvents (e.g., DMSO, DMF); therefore, the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.
Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores, which is compatible with various instruments. Cyanine5 can also be used as a replacement for DyLight® 649.
Absorbance and emission spectra of Cyanine5
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Cyanine3.5 DBCO
Cyclooctyne derivative of Cyanine3.5 dye, which easily reacts with various azides resulting in stable conjugates.sulfo-Cyanine5 amine
Sulfonated Cyanine5 amine derivative for the conjugation with electrophiles and enzymatic transamination labeling.General properties
Appearance: | dark blue powder / solution |
Molecular weight: | 601.22 |
CAS number: | 1267539-32-1 (chloride) |
Molecular formula: | C35H45ClN6O |
Solubility: | soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L) |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 646 |
ε, L⋅mol−1⋅cm−1: | 250000 |
Emission maximum, nm: | 662 |
Fluorescence quantum yield: | 0.2 |
CF260: | 0.03 |
CF280: | 0.04 |
Product citations
- Xu, M.J.; Jordan, P.W. SMC5/6 Promotes Replication Fork Stability via Negative Regulation of the COP9 Signalosome. International Journal of Molecular Sciences, 2024, 25(2), 952. doi: 10.3390/ijms25020952
- Ramezani, M.; Bauman, J.; Singh, A.; Weisbart, E.; Yong, J.; Lozada, M.; Way, G.P.; Kavari, S.L.; Diaz, C.; Haghighi, M.; Batista, T.M.; Pérez-Schindler, J.; Claussnitzer, M.; Singh, S.; Cimini, B.A.; Blainey, P.C.; Carpenter, A.E.; Jan, C.H.; Neal, J.T. A genome-wide atlas of human cell morphology. bioRxiv, 2023. doi: 10.1101/2023.08.06.552164
- Tsuchiya, M.; Tachibana, N.; Hamachi, I. Flow cytometric analysis of phosphatidylcholine metabolism using organelle-selective click labeling. STAR Protocols, 2023, 4(3), 102525. doi: 10.1016/j.xpro.2023.102525
- Zhao, Y.; Miranda Herrera, P. A.; Chang, D.; Hamelin, R.; Long, M. J. C.; Aye, Y. Function-Guided Proximity Mapping Unveils Electrophilic-Metabolite Sensing by Proteins Not Present in Their Canonical Locales. Proc. Natl. Acad. Sci. U.S.A., 2022, 119(5), e2120687119. doi: 10.1073/pnas.2120687119