Cyanine5 azide

Azides Cyanine5 Click chemistry
Cat. # Quantity Price Lead time
13030 100 uL, 10 mM/DMSO –   in stock
33030 500 uL, 10 mM/DMSO $199 in stock
43030 1 mL, 10 mM/DMSO $495 in stock
A3030 1 mg –   in stock
B3030 5 mg $199 in stock
C3030 10 mg $297 in stock
D3030 25 mg $495 in stock
E3030 50 mg $895 in stock
F3030 100 mg $1490 in stock
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Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO and in solid form.

This azide is soluble in organic solvents (e.g., DMSO, DMF); therefore, the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.

Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores, which is compatible with various instruments. Cyanine5 can also be used as a replacement for DyLight® 649.

Cyanine5 azide, D3030
Cyanine5 azide, D3030
Cyanine5 azide, D3030
Cyanine5 azide, azide stock solution for click chemistry (10 mM in DMSO), 43030
Cyanine5 azide, azide stock solution for click chemistry (10 mM in DMSO), 43030
Cyanine5 azide, azide stock solution for click chemistry (10 mM in DMSO), 43030
Cyanine5 azide, azide stock solution for click chemistry (10 mM in DMSO), 43030
Structure of Cyanine5 azide
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Absorbance and emission spectra of Cyanine5

Absorbance and emission spectra of Cyanine5

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AF 488 DBCO

Dibenzocyclooctyne (DBCO, ADIBO) containing the green fluorophore AF 488 for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples. Pure 5-isomer.

General properties

Appearance: dark blue powder / solution
Molecular weight: 601.22
CAS number: 1267539-32-1 (chloride)
Molecular formula: C35H45ClN6O
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Xu, M.J.; Jordan, P.W. SMC5/6 Promotes Replication Fork Stability via Negative Regulation of the COP9 Signalosome. International Journal of Molecular Sciences, 2024, 25(2), 952. doi: 10.3390/ijms25020952
  2. Ryl, T.; Afanasyeva, E.; Hartmann, T.; Schwermer, M.; Schneider, M.; Schröder, C.; Wagemanns, M.; Bister, A.; Kanber, D.; Steenpass, L.; Schramm, K.; Jones, B.; Jones, D.T.W.; Biewald, E.; Astrahantseff, K.; Hanenberg, H.; Rahmann, S.; Lohmann, D.R.; Schramm, A.; Ketteler, P. A MYCN-driven de-differentiation profile identifies a subgroup of aggressive retinoblastoma. Communications Biology, 2024, 7, 919. doi: 10.1038/s42003-024-06596-6
  3. Ramezani, M.; Bauman, J.; Singh, A.; Weisbart, E.; Yong, J.; Lozada, M.; Way, G.P.; Kavari, S.L.; Diaz, C.; Haghighi, M.; Batista, T.M.; Pérez-Schindler, J.; Claussnitzer, M.; Singh, S.; Cimini, B.A.; Blainey, P.C.; Carpenter, A.E.; Jan, C.H.; Neal, J.T. A genome-wide atlas of human cell morphology. bioRxiv, 2023. doi: 10.1101/2023.08.06.552164
  4. Tsuchiya, M.; Tachibana, N.; Hamachi, I. Flow cytometric analysis of phosphatidylcholine metabolism using organelle-selective click labeling. STAR Protocols, 2023, 4(3), 102525. doi: 10.1016/j.xpro.2023.102525
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Cy® is a trademark of Cytiva.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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