DiD, lipophilic tracer

Neuroscience Flow cytometry Microscopy Cell membrane
Cat. # Quantity Price Lead time
3251-1mg 1 mg –   in stock
3251-5mg 5 mg $56 in stock
3251-50mg 50 mg $240 in stock
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DiD is a far-red fluorescent carbocyanine dye for cell membrane labeling in vivo and in vitro. DiD diffuses laterally to stain the entire cell, allowing it to be used as an anterograde and retrograde tracer of neurons. In intact tissue, the dye does not transfer from labeled to unlabeled cells, but some transfer may occur when the membrane is disrupted, for example, after sectioning. The dye is weakly fluorescent until incorporated into membranes.

DiD can be used with other tracers in dual-color studies, such as DiI or DiO.

DiD is a solid form for the direct application of crystals to membranes.

DiD, lipophilic tracer, 3251-50mg
DiD, lipophilic tracer, 3251-50mg
DiD, lipophilic tracer, 3251-50mg
Structure of DiD, lipophilic tracer
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Product in action

Human peripheral blood neutrophils stained with DiD (3251-x) lipophilic tracer and DAPI (2996-x) nuclear stain

Absorption and emission spectra of DiD

Absorption and emission spectra of DiD

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General properties

Appearance: dark blue solid
Molecular weight: 987.38
CAS number: 75539-51-4
Molecular formula: C61H99IN2
Solubility: DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 647
Emission maximum, nm: 666

Product citations

  1. Sindeeva, O.A.; Demina, P.A.; Kozyreva, Z.V.; Terentyeva, D.A.; Gusliakova, O.I.; Muslimov, A.R.; Sukhorukov, G.B. Single Mesenchymal Stromal Cell Migration Tracking into Glioblastoma Using Photoconvertible Vesicles. Nanomaterials, 2024, 14(14), 1215. doi: 10.3390/nano14141215
  2. Liu, X.; Cao, Z.; Liu, N.; Gao, G.; Du, M.; Wang, Y.; Cheng, B.; Zhu, M.; Jia, B.; Pan, L.; Zhang, W.; Jiang, Y.; He, W.; Xu, L.; Zhang, W.; An, Q.; Guo, Q.; Gu, J. Kill two birds with one stone: Engineered exosome-mediated delivery of cholesterol modified YY1-siRNA enhances chemoradiotherapy sensitivity of glioblastoma. Frontiers in Pharmacology, 2022, 13, 975291. doi: 10.3389/fphar.2022.975291
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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