AF 430 amine

Cat. # Quantity Price Lead time
108C0 1 mg $125 in stock
208C0 5 mg $325 in stock
408C0 25 mg $950 in stock
508C0 50 mg $1625 in stock
608C0 100 mg $2090 in stock
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AF 430 is a stable dye over a broad pH range (from 4 to 10) with the fluorescence peak in the yellow-green region (at 542 nm).

Conjugates of biomolecules with AF 430 can be used in flow cytometry, for example in multicolor staining. AF 430 is also used in cell microscopy due to its high photostability.

AF 430 amine is soluble in water and can be conjugated with electrophiles and participate in enzymatic transamination.

Absorption and emission spectra of AF 430

Absorption and emission spectra of AF 430

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EDC (N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide), coupling grade

Water soluble carbodiimide for the coupling of carboxylic acids with amines.

General properties

Appearance: yellow solid
Molecular weight: 601.68
Molecular formula: C28H38N3F3O6S
IUPAC name: (9-(6-((6-ammoniohexyl)amino)-6-oxohexyl)-8,8-dimethyl-2-oxo-4-(trifluoromethyl)-8,9-dihydro-2H-pyrano[3,2-g]quinolin-6-yl)methanesulfonate
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 430
ε, L⋅mol−1⋅cm−1: 15955
Emission maximum, nm: 542
Fluorescence quantum yield: 0.23
CF260: 0.06
CF280: 0.06
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