VIC azide, 6-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
1H230 | 1 mg | $110 | in stock | |
2H230 | 5 mg | $210 | in stock | |
4H230 | 25 mg | $410 | in stock | |
5H230 | 50 mg | $695 | in stock | |
6H230 | 100 mg | $1190 | in stock |
VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE. Oligonucleotides labeled with VIC are often used in real-time PCR; the preparation of such oligonucleotides can be performed using click chemistry. This derivative is an azide, a pure 6-isomer, for conjugating VIC to other molecules by copper-catalyzed and copper-free click reactions.
Absorption and emission spectra of 6-VIC
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Ascorbic acid is a reducing agent converting copper(II) into a monovalent form that catalyzes the click reaction.AF 555 NHS ester
AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.General properties
Appearance: | orange powder |
Molecular weight: | 637.86 |
Molecular formula: | C30H19Cl3N4O6 |
Solubility: | very soluble in DMSO, DMF |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 525 |
ε, L⋅mol−1⋅cm−1: | 103000 |
Emission maximum, nm: | 546 |
Fluorescence quantum yield: | 0.53 |
CF260: | 0.07 |
CF280: | 0.07 |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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