AMCA carboxylic acid

Cat. # Quantity Price Lead time
3357-25mg 25 mg $45 in stock
3357-100mg 100 mg $95 in stock
3357-1g 1 g $490 in stock
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AMCA (aminomethylcoumarin acetate) is one of the brightest blue fluorescent dyes. This fluorophore has a relatively large Stoke’s shift, high resistance to photobleaching, and pH-independent fluorescence from pH 4 to 10. AMCA is a widely used fluorophore for multiple-color labeling due to its minimal fluorescence overlap with green- and longer wavelength-emitting fluorescent dyes.

AMCA carboxylic acid is a non-reactive form of AMCA dye that can be used as a reference standard in experiments involving AMCA dye conjugates. Besides, the carboxylic group can react with hydrazines, hydroxylamines, and amines using carbodiimides such as EDAC.

Absorption and emission spectra of AMCA

Absorption and emission spectra of AMCA

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General properties

Appearance: off-white solid
Molecular weight: 233.22
Molecular formula: C12H11NO4
Solubility: good in DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 348
ε, L⋅mol−1⋅cm−1: 17400
Emission maximum, nm: 435
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.13
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