AF 555 DBCO

Cat. # Quantity Price Lead time
1K8F0 1 mg $140 in stock
2K8F0 5 mg $450 in stock
4K8F0 25 mg $1460 in stock
5K8F0 50 mg $1890 in stock
6K8F0 100 mg $2990 in stock
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Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.

AF 555 is a hydrophilic fluorophore with high fluorescence quantum yield and high photostability, an alternative to tetramethylrhodamine (TAMRA, TMR) or Cyanine3 dyes.

AF 555 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.

Absorption and emission spectra of AF 555

Absorption and emission spectra of AF 555

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General properties

Appearance: purple powder
Molecular weight: 1279.71
Molecular formula: C56H65K3N4O15S4
Solubility: water, DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 552
ε, L⋅mol−1⋅cm−1: 152000
Emission maximum, nm: 566
Fluorescence quantum yield: 0.14
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