DiI, lipophilic tracer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3225-1mg | 1 mg | – | in stock | |
| 3225-5mg | 5 mg | $24 | in stock | |
| 3225-25mg | 25 mg | $110 | in stock | |
| 3225-100mg | 100 mg | $238 | in stock |
DiI (DiIC18(3); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) is an orange-red fluorescent carbocyanine dye.
DiI is a widely used lipophilic dye that labels cell membranes by inserting its two long hydrocarbon (C18 carbon) chains into the lipid bilayer. The dye is weakly fluorescent until incorporated into membranes. DiI diffuses laterally to stain the entire cell, allowing it to be used as an anterograde and retrograde tracer of neurons. In intact tissue, the dye does not transfer from labeled to unlabeled cells, but some transfer may occur when the membrane is disrupted, for example, after sectioning.
DiI is often used with other tracers in dual-color studies, such as DiA and DiO.
Product in action
Tracing of neurons with lipophilic tracer DiI (3225-x) in the mouse brain
Tracing of triangular septal nucleus neurons with lipophilic tracer DiI (3225-x) in the mouse brain
Absorption and emission spectra of DiI
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| Appearance: | violet solid |
| Molecular weight: | 961.34 |
| CAS number: | 22366-93-4 |
| Molecular formula: | C59H97IN2 |
| Solubility: | DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 551 |
| ε, L⋅mol−1⋅cm−1: | 144500 |
| Emission maximum, nm: | 566 |
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