AF 384 (PEP) azide

Cat. # Quantity Price Lead time
A4530 1 mg $110 in stock
B4530 5 mg $210 in stock
C4530 10 mg $310 in stock
D4530 25 mg $410 in stock
E4530 50 mg $695 in stock
Found better price? Let us know and we will propose the way forward!

Phenylethynylpyrene (PEP) fluorophore is a polyaromatic hydrocarbon label with high sensitivity to microenvironment. Similarly to pyrene, PEP dye readily forms excimers. However, AF 384 (PEP) fluorescence is more red-shifted

PEP can be used as microenvironment probe, and as a label for assays based on excimer formation.

This reagent contains triethyleneglycol linker to facilitate dissolution of non-polar PEP dye in organo-aqueous labeling reaction mixtures. With this azide, and click chemistry, it is easy to turn any molecule bearing alkyne into PEP-labeled probe.

Spectra of AF 384 (PEP) (phenylethynylpyrene) in ethanol

Spectra of AF 384 (PEP) (phenylethynylpyrene) in ethanol

Customers also purchased with this product

AF 343 (Coumarin)-X-azide

AF 343 (Coumarin) is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by сlick chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility and provides spatial separation between the dye and the biomolecule.

AF 555 NHS ester

AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.

BDP® 630/650-X-NHS ester

BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays. This is an amine-reactive NHS ester derivative.

General properties

Appearance: off white / yellowish solid
Molecular weight: 544.64
CAS number: 1807521-02-3
Molecular formula: C34H32N4O3
Solubility: soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile
Quality control: NMR 1H (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 293; 362; 384
ε, L⋅mol−1⋅cm−1: 46300
Emission maximum, nm: 389
Fluorescence quantum yield: 0.64

Product citations

  1. Taskova, M.; Astakhova, K. Fluorescent Oligonucleotides with Bis(prop-2-yn-1-yloxy)butane-1,3-diol Scaffold Rapidly Detect Disease-Associated Nucleic Acids. Bioconjugate Chemistry, 2019, 30(12), 3007–3012. doi: 10.1021/acs.bioconjchem.9b00746
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.