AF 384 (PEP) azide
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
A4530 | 1 mg | $110 | in stock | |
B4530 | 5 mg | $210 | in stock | |
C4530 | 10 mg | $310 | in stock | |
D4530 | 25 mg | $410 | in stock | |
E4530 | 50 mg | $695 | in stock |
Phenylethynylpyrene (PEP) fluorophore is a polyaromatic hydrocarbon label with high sensitivity to microenvironment. Similarly to pyrene, PEP dye readily forms excimers. However, AF 384 (PEP) fluorescence is more red-shifted
PEP can be used as microenvironment probe, and as a label for assays based on excimer formation.
This reagent contains triethyleneglycol linker to facilitate dissolution of non-polar PEP dye in organo-aqueous labeling reaction mixtures. With this azide, and click chemistry, it is easy to turn any molecule bearing alkyne into PEP-labeled probe.
Spectra of AF 384 (PEP) (phenylethynylpyrene) in ethanol
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Appearance: | off white / yellowish solid |
Molecular weight: | 544.64 |
CAS number: | 1807521-02-3 |
Molecular formula: | C34H32N4O3 |
Solubility: | soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile |
Quality control: | NMR 1H (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 293; 362; 384 |
ε, L⋅mol−1⋅cm−1: | 46300 |
Emission maximum, nm: | 389 |
Fluorescence quantum yield: | 0.64 |
Product citations
- Taskova, M.; Astakhova, K. Fluorescent Oligonucleotides with Bis(prop-2-yn-1-yloxy)butane-1,3-diol Scaffold Rapidly Detect Disease-Associated Nucleic Acids. Bioconjugate Chemistry, 2019, 30(12), 3007–3012. doi: 10.1021/acs.bioconjchem.9b00746