DusQ® 1 dT phosphoramidite

Oligonucleotide synthesis DusQ® 1 Phosphoramidites

Cat. #	Quantity	Price	Lead time
1452-100mg	100 mg	$340	in stock
1452-500mg	500 mg	$1390	in stock
1452-1g	1 g	$2290	in stock

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DusQ 1 dT phosphoramidite is a modified oligo synthesis reagent bearing true dark quencher nucleotide base typically used to internally label DNA oligonucleotide probe. DusQ 1 efficiently quench fluorescence by FRET quenching and through static quenching via formation of a ground state complex with the reporter dye, and typically used to construct qPCR probes with a quencher moiety.

Reagent contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges. DusQ 1 can be paired with reporter dyes emitting in the green to yellow region to form completely non-fluorescent combination. DusQ 1 has a broad absorption spectrum with maximum in a range 480–580 nm and works in conjunction with the commonly used fluorophores, e.g. FAM, TET, JOE, HEX, and Cyanine3.

Usage

Coupling: 6 minutes coupling time recommended.

Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).

Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.

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Absorption spectrum of DusQ 1

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C8-Alkyne-dT-CE-phosphoramidite

C8-Alkyne-dT-CE-phosphoramidite is a specialized reagent used in the synthesis of oligonucleotides that incorporates a single dT alkyne group. This compound is particularly valuable for applications involving click chemistry, allowing for efficient conjugation reactions.

ROX azide, 5-isomer

Azide derivative of ROX (Rhodamine X, Rhodamine 101) dye for biomolecule labeling via click chemistry. Pure 5-isomer.

General properties

Appearance:	black powder
Molecular weight:	1411.58
Molecular formula:	C₇₆H₉₁N₁₂O₁₃P
Quality control:	NMR ¹H, ³¹P,HPLC-MS (95%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	522
ε, L⋅mol⁻¹⋅cm⁻¹:	27300

Oligo synthesis details

Coupling conditions:	6 min coupling time
Cleavage conditions:	ammonia, 2 h at room temperature
Deprotection conditions:	identical to protected nucleobases

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.