DusQ 2 dT phosphoramidite
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
1451-100mg | 100 mg | $340 | in stock | |
1451-500mg | 500 mg | $1390 | in stock | |
1451-1g | 1 g | $2290 | in stock |
DusQ 2 dT phosphoramidite is a modified oligo synthesis reagent bearing completely non-fluorescent quencher nucleotide base typically used to internally label DNA oligonucleotide probe. DusQ 2 has large extinction coefficient, efficiently quench fluorescence for dyes emitting in the far red region, such as Cyanine5.
DusQ 2 is typically used to construct qPCR probes with a quencher moiety. Reagent contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges. DusQ 2 has a absorption spectrum in of red and near-infrared range and can be paired with reporter dyes that emit in the range 560–670 nm, e.g. TAMRA, ROX, Cyanine3, and Cyanine5.
Usage
Coupling: 6 minutes coupling time recommended.
Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).
Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.
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Appearance: | black powder |
Molecular weight: | 1413.55 |
Molecular formula: | C75H89N12O14P |
Quality control: | NMR 1H, 31P,HPLC-MS (95%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 552 |
Oligo synthesis details
Coupling conditions: | 6 min coupling time |
Cleavage conditions: | ammonia, 2 h at room temperature |
Deprotection conditions: | identical to protected nucleobases |