Biotin-11-dUTP

PCR and qPCR Triphosphates

Cat. #	Quantity	Price	Lead time
1553-250nmol	250 nmol	$140	20 days
1553-500nmol	500 nmol	$235	in stock
1553-1umol	1 umol	$360	in stock
1553-5umol	5 umol	please inquire	in stock

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Biotinylated deoxyuridine triphosphate (dUTP) can be used for DNA labeling using various methods, including Nick-translation, polymerase chain reaction (PCR), random priming, and 3’-terminal non-radioactive labeling. Generated probes can be used in various hybridization experiments such as Southern blot, Northern blot, dot blot, or FISH. Biotin-labeled probes are detected with horseradish peroxidase-conjugated streptavidin or other biomolecule conjugates binding biotin.

«11» in the compound name indicates the length of the linker between dUTP and biotin; this linker increases the efficiency of labeled dUTP incorporation into DNA and interaction of biotin with specific proteins such as avidin/streptavidin or anti-biotin antibodies.

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General properties

Appearance:	colorless solid
Molecular weight:	878.54
Molecular formula:	C₂₈H₄₀N₆Li₃O₁₇P₃S
IUPAC name:	((2R,3S,5R)-5-(2,4-dioxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility:	soluble in water
Quality control:	HPLC-MS (95%), testing in enzymatic reaction
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles.
MSDS:	Download
	Product specifications

Product citations

Krasikova, A.; Kulikova, T.; Schelkunov, M.; Makarova, N.; Fedotova, A.; Berngardt, V.; Maslova, A.; Fedorov, A. The first chicken oocyte nucleus whole transcriptomic profile defines the spectrum of maternal mRNA and non-coding RNA genes transcribed by the lampbrush chromosomes. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.05.577752
Krasikova, A.; Kulikova, T.; Rodriguez Ramos, J. S.; Maslova, A. Assignment of the Somatic A/B Compartments to Chromatin Domains in Giant Transcriptionally Active Lampbrush Chromosomes. Epigenetics & Chromatin, 2023, 16(1), 24. doi: 10.1186/s13072-023-00499-2