Biotin-X-NHS ester

Cat. # Quantity Price Lead time
2551-50mg 50 mg –   in stock
2551-100mg 100 mg $117 in stock
2551-1g 1 g $450 in stock
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Biotin may be conjugated to many proteins while maintaining the biological activity. The biotinylated probe is usually detected by avidin, streptavidin due to their effective binding which is used in various applications such as affinity chromatography, ELISA and Western Blot, fluorescence-activated cell sorting (FACS), intracellular labeling.

Biotin-X-NHS ester is a derivative with C6-spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines. This compound is used to attach biotin to primary amines under alkaline conditions (pH 8-9).

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General properties

Appearance: white solid
Mass spec M+ increment: 339.2
Molecular weight: 454.55
CAS number: 72040-63-2
Molecular formula: C20H30N4O6S
IUPAC name: (2,5-dioxopyrrolidin-1-yl) 6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoate
Solubility: good in DMSO, moderately soluble in DMF
Quality control: NMR 1H
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Product citations

  1. Rak, A.; Matyushenko, V.; Prokopenko, P.; Kostromitina, A.; Polyakov, D.; Sokolov, A.; Rudenko, L.; Isakova-Sivak, I. A novel immunofluorescent test system for SARS-CoV-2 detection in infected cells. PLOS ONE, 2024, 19(5), e0304534. doi: 10.1371/journal.pone.0304534
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