Biotinyl tyramide

Biotinylation Microscopy Tyramides

Cat. #	Quantity	Price	Lead time	Buy this product
4903-100mg	100 mg	$105	12 days
4903-500mg	500 mg	$439	12 days

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Tyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.

Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.

This tyramide is a biotin derivative for tyramide amplification and subsequent signal detection with a labeled streptavidin conjugate. It can be used with any antibody or other molecules conjugated to HRP to stain cells and tissues by immunofluorescence methods.

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Tyramide Signal Amplification (TSA)

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General properties

Appearance:	white solid
Molecular weight:	363.48
CAS number:	41994-02-9
Molecular formula:	C₁₈H₂₅N₃O₃S
Solubility:	DMSO, DMF, methanol
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.