Cyanine5 maleimide

Cat. # Quantity Price Lead time
13080 1 mg $125 in stock
23080 5 mg $260 in stock
43080 25 mg $510 in stock
53080 50 mg $895 in stock
63080 100 mg $1490 in stock
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Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.

Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.

For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.

Cyanine5 excitation and emission spectra

Cyanine5 excitation and emission spectra

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sulfo-Cyanine3 tyramide

A tyramide derivative of sulfo-Cyanine3, an orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

R6G NHS ester, 5-isomer

R6G (rhodamine 6G) NHS ester, pure 5-isomer. This dye is often used for the labeling of oligonucleotides for qPCR.

General properties

Appearance: dark blue powder
Molecular weight: 605.8
CAS number: 1437872-46-2 (without anion)
Molecular formula: C38H45N4O3
IUPAC name: 3H-​Indolium, 2-​[5-​[1-​[6-​[[2-​(2,​5-​dihydro-​2,​5-​dioxo-​1H-​pyrrol-​1-​yl)​ethyl]​amino]​-​6-​oxohexyl]​-​1,​3-​dihydro-​3,​3-​dimethyl-​2H-​indol-​2-​ylidene]​-​1,​3-​pentadien-​1-​yl]​-​1,​3,​3-​trimethyl-
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Frazier, C.L.; Deb, D.; Weeks, A.M. Engineered reactivity of a bacterial E1-like enzyme enables ATP-driven modification of protein C termini. bioRxiv, 2024, preprint. doi: 10.1101/2024.05.13.593989
  2. Huang, L.; Liu, P.; Du, Y.; Pan, D.; Lee, A.; Wolfe, S.A.; Wang, Y.-X. A brown fat-enriched adipokine, ASRA, is a leptin receptor antagonist that stimulates appetite. bioRxiv, 2024, preprint. doi: 10.1101/2023.09.12.557454
  3. Johnson, J.L.; Steele, J.H.; Lin, R.; Stepanov, V.G.; Gavriliuc, M.N.; Wang, Y. Multi-Channel smFRET study reveals a Compact conformation of EF-G on the Ribosome. bioRxiv, 2024, preprint. doi: 10.1101/2024.01.27.577133
  4. Ernst, M.; Orabi, E.A.; Stockbridge, R.B.; Faraldo-Gómez, J.D.; Robertson, J.L. Dimerization mechanism of an inverted-topology ion channel in membranes. Proceedings of the National Academy of Sciences of the United States of America, 2024, 120(47), e2308454120. doi: 10.1073/pnas.2308454120
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