Cyanine5 maleimide
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
13080 | 1 mg | $125 | in stock | |
23080 | 5 mg | $260 | in stock | |
43080 | 25 mg | $510 | in stock | |
53080 | 50 mg | $895 | in stock | |
63080 | 100 mg | $1490 | in stock |
Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.
Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.
For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.
Cyanine5 excitation and emission spectra
Recommended protocol
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Orange-red fluorescent lipophilic dye for membrane labeling and cell tracing.General properties
Appearance: | dark blue powder |
Molecular weight: | 605.8 |
CAS number: | 1437872-46-2 (without anion) |
Molecular formula: | C38H45N4O3 |
IUPAC name: | 3H-Indolium, 2-[5-[1-[6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-trimethyl- |
Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L) |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 646 |
ε, L⋅mol−1⋅cm−1: | 250000 |
Emission maximum, nm: | 662 |
Fluorescence quantum yield: | 0.2 |
CF260: | 0.03 |
CF280: | 0.04 |
Product citations
- Frazier, C.L.; Deb, D.; Weeks, A.M. Engineered reactivity of a bacterial E1-like enzyme enables ATP-driven modification of protein C termini. bioRxiv, 2024, preprint. doi: 10.1101/2024.05.13.593989
- Johnson, J.L.; Steele, J.H.; Lin, R.; Stepanov, V.G.; Gavriliuc, M.N.; Wang, Y. Multi-Channel smFRET study reveals a Compact conformation of EF-G on the Ribosome. bioRxiv, 2024, preprint. doi: 10.1101/2024.01.27.577133
- Uckelmann, M.; Levina, V.; Taveneau, C.; Han Ng, X.; Pandey, V.; Martinez, J.; Mendiratta, S.; Houx, J.; Boudes, M.; Venugopal, H.; Trépout, S.; Zhang, Q.; Flanigan, S.; Li, M.; Sierecki, E.; Gambin, Y.; Das, P.P.; Bell, O.; de Marco, A.; Davidovich, C. Dynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates. bioRxiv, 2024, preprint. doi: 10.1101/2023.05.08.539931
- Ernst, M.; Orabi, E.A.; Stockbridge, R.B.; Faraldo-Gómez, J.D.; Robertson, J.L. Dimerization mechanism of an inverted-topology ion channel in membranes. Proceedings of the National Academy of Sciences of the United States of America, 2024, 120(47), e2308454120. doi: 10.1073/pnas.2308454120