R6G NHS ester, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14220 | 1 mg | $110 | in stock | |
| 24220 | 5 mg | $210 | in stock | |
| 44220 | 25 mg |
$410
|
in stock | |
| 54220 | 50 mg |
$695
|
in stock | |
| 64220 | 100 mg |
$1190
|
in stock |
Rhodamine 6G (R6G) is a xanthene dye of rhodamine series that has been used for the labeling of oligonucleotides and DNA for quite a long time. Just like other xanthenes, R6G comes as two isomers, 5- and 6-isomer. Even though their absorption and emission spectra are virtually indistinguishable, the isomers need to be separated to avoid separation of the labeled molecules during their purification, i.e. double HPLC peaks and double spots on electrophoresis.
This is an amine reactive NHS ester derived from a pure 5-isomer of R6G. Rhodamine 6G is very bright (even used as a quantum yield standard, yield 0.95). The dye is soluble in organic solvents, such as DMF, or DMSO. They can be used as co-solvents for the labeling reactions.
Absorption and emission spectra for 5-R6G
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EdU (5-Ethynyl-2'-deoxyuridine)
EdU is a nucleoside that is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by click chemistry reaction with fluorescent dye azides to reveal cell proliferation.General properties
| Appearance: | dark colored solid |
| Molecular weight: | 555.58 |
| Molecular formula: | C31H29N3O7 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 519 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 546 |
| Fluorescence quantum yield: | 0.95 |
| CF260: | 0.18 |
| CF280: | 0.17 |
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