Cyanine3.5 DBCO
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A20F0 | 1 mg | $125 | in stock | |
| B20F0 | 5 mg | $260 | in stock | |
| C20F0 | 10 mg | $325 | in stock | |
| D20F0 | 25 mg |
$510
|
in stock | |
| E20F0 | 50 mg |
$895
|
in stock | |
| F20F0 | 100 mg |
$1490
|
in stock |
Cyanine3.5 is a bright and photostable fluorophore, which is similar in its spectral characteristics to Cy3.5™ dye. Cyanine3.5 DBCO fluorescence maximum is at 604 nm and in the orange-red spectrum range.
Cyanine3.5 fluorescence is pH insensitive in the range from 4 to 10 and can be detected in the spectrum range with a low level of autofluorescence of biological samples.
Cyanine3.5 DBCO (dibenzocyclooctyne) easily forms conjugates with azide derivatives of biomolecules in copper-free Click Chemistry. The process runs without catalysts or increased temperature, resulting in stable triazoles.
Conjugates of biomolecules with Cyanine3.5 can be used for various microscopy assays, including FRET-microscopy, and in proteomics.
Absorption and emission spectra of Cyanine3.5
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A dye with a dibenzocyclooctyne substituting group for the ROX channel.General properties
| Appearance: | dark violet solid |
| Molecular weight: | 944.95 |
| Molecular formula: | C59H61N4BF4O2 |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 591 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 604 |
| Fluorescence quantum yield: | 0.35 |
Cy™ is a registered trademark of Cytiva in some countries.
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