Cyanine3 phosphoramidite

Cat. # Quantity Price Lead time
2771-100mg 100 mg –   in stock
2771-250mg 250 mg $290 in stock
2771-1g 1 g $950 in stock
2771-10g 10 g please inquire in stock
Found better price? Let us know and we will propose the way forward!

Cyanine3 is a fluorophore that is widely used in molecular biology experiments such as oligonucleotide labeling followed by oligonucleotide detection. By its spectral characteristics, Cyanine3 is a dye with a fluorescence maximum at 570 nm in the yellow spectrum range.

Cyanine3 phosphoramidite 5’ is used in oligonucleotide synthesis for the production of 5’-cyanine3-labeled oligonucleotides. The reagent is compatible with various oligonucleotide synthesizers.

This phosphoramidite can be used for the synthesis of fluorescence-labeled primers and hybridization probes such as TaqMan and Molecular Beacon. Such labeled probes can be detected in multiplex real-time PCR in the TAMRA channel.

Usage:

Condensation: 3 min. Use 0.02 M iodine solution at the oxidation step to avoid degradation of the cyanine dye.

Deprotection: At room temperature with 30% aqueous ammonium solution. It is recommended to use nucleic bases with labile protective groups for deprotection for not more than 2 h at less than 55°C. AMA (30% aqueous ammonium solution/40% aqueous methylamine 1:1 (v/v)) can be used for 10 min at 65°C in the presence of acetyldeoxycytidine. If deoxyguanidine with a dimethylformamidine protective group is used during synthesis, deprotect with 30% aqueous ammonium solution for 2 h at 65°C. If deoxyguanidine with an isobutyryl protective group is used during synthesis, deprotect for 24-36 h at room temperature.

Customers also purchased with this product

DiD, lipophilic tracer

Far-red fluorescent lipophilic dye for membrane labeling and cell tracing.

Cyanine5.5 amine

Amino derivative of Cyanine5.5, a far red emitting fluorescent cyanine dye.
Add this product to your cart and
get free express delivery

Cyanine5 phosphoramidite

Phosphoramidite derivative of Cyanine5 for use in oligonucleotide synthesis.

General properties

Appearance: red powder
Molecular weight: 841.81
Molecular formula: C45H65N5BF4O3P
Solubility: good in acetonitrile, dichloromethane
Quality control: NMR 1H, 31P, HPLC-MS (80%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.