Cyanine3 phosphoramidite
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2771-100mg | 100 mg | – | in stock | |
| 2771-250mg | 250 mg | $290 | in stock | |
| 2771-1g | 1 g |
$950
|
in stock | |
| 2771-10g | 10 g | please inquire | in stock |
Cyanine3 is a fluorophore that is widely used in molecular biology experiments such as oligonucleotide labeling followed by oligonucleotide detection. By its spectral characteristics, Cyanine3 is a dye with a fluorescence maximum at 570 nm in the yellow spectrum range.
Cyanine3 phosphoramidite 5’ is used in oligonucleotide synthesis for the production of 5’-cyanine3-labeled oligonucleotides. The reagent is compatible with various oligonucleotide synthesizers.
This phosphoramidite can be used for the synthesis of fluorescence-labeled primers and hybridization probes such as TaqMan and Molecular Beacon. Such labeled probes can be detected in multiplex real-time PCR in the TAMRA channel.
Usage:
Condensation: 3 min. Use 0.02 M iodine solution at the oxidation step to avoid degradation of the cyanine dye.
Deprotection: At room temperature with 30% aqueous ammonium solution. It is recommended to use nucleic bases with labile protective groups for deprotection for not more than 2 h at less than 55°C. AMA (30% aqueous ammonium solution/40% aqueous methylamine 1:1 (v/v)) can be used for 10 min at 65°C in the presence of acetyldeoxycytidine. If deoxyguanidine with a dimethylformamidine protective group is used during synthesis, deprotect with 30% aqueous ammonium solution for 2 h at 65°C. If deoxyguanidine with an isobutyryl protective group is used during synthesis, deprotect for 24-36 h at room temperature.
Customers also purchased with this product
PODi-1, blue fluorescent nucleic acid stain
PODi-1 is a cell-impermeant blue fluorescent nucleic acid stain. PODi-1 is chemically equivalent to POPO®-1.SynaptoProbe® Green
SynaptoProbe® Green is an analog of FM®1-43, a widely used green fluorescent dye for staining synaptic vesicles and studying synaptic activity in the synapses or neuromuscular junctions.Cyanine7 DBCO
NIR fluorescent dye functionalized with cyclooctyne moiety for copper free click chemistry conjugation with azides.
Add this product to your cart andget free express delivery
General properties
| Appearance: | red powder |
| Molecular weight: | 841.81 |
| Molecular formula: | C45H65N5BF4O3P |
| Solubility: | good in acetonitrile, dichloromethane |
| Quality control: | NMR 1H, 31P, HPLC-MS (80%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | 6 min coupling time recommended |
| Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |
Your cart is empty
$ 
