Cyanine3 phosphoramidite
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
2771-100mg | 100 mg | – | in stock | |
2771-250mg | 250 mg | $290 | in stock | |
2771-1g | 1 g | $950 | in stock | |
2771-10g | 10 g | please inquire | in stock |
Cyanine3 is a fluorophore that is widely used in molecular biology experiments such as oligonucleotide labeling followed by oligonucleotide detection. By its spectral characteristics, Cyanine3 is a dye with a fluorescence maximum at 570 nm in the yellow spectrum range.
Cyanine3 phosphoramidite 5’ is used in oligonucleotide synthesis for the production of 5’-cyanine3-labeled oligonucleotides. The reagent is compatible with various oligonucleotide synthesizers.
This phosphoramidite can be used for the synthesis of fluorescence-labeled primers and hybridization probes such as TaqMan and Molecular Beacon. Such labeled probes can be detected in multiplex real-time PCR in the TAMRA channel.
Usage:
Condensation: 3 min. Use 0.02 M iodine solution at the oxidation step to avoid degradation of the cyanine dye.
Deprotection: At room temperature with 30% aqueous ammonium solution. It is recommended to use nucleic bases with labile protective groups for deprotection for not more than 2 h at less than 55°C. AMA (30% aqueous ammonium solution/40% aqueous methylamine 1:1 (v/v)) can be used for 10 min at 65°C in the presence of acetyldeoxycytidine. If deoxyguanidine with a dimethylformamidine protective group is used during synthesis, deprotect with 30% aqueous ammonium solution for 2 h at 65°C. If deoxyguanidine with an isobutyryl protective group is used during synthesis, deprotect for 24-36 h at room temperature.
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Cyanine5 phosphoramidite
Phosphoramidite derivative of Cyanine5 for use in oligonucleotide synthesis.General properties
Appearance: | red powder |
Molecular weight: | 841.81 |
Molecular formula: | C45H65N5BF4O3P |
Solubility: | good in acetonitrile, dichloromethane |
Quality control: | NMR 1H, 31P, HPLC-MS (80%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 555 |
ε, L⋅mol−1⋅cm−1: | 150000 |
Emission maximum, nm: | 570 |
Fluorescence quantum yield: | 0.31 |
CF260: | 0.04 |
CF280: | 0.09 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | 6 min coupling time recommended |
Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |