RAPID DiO, lipophilic tracer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3708-500ug | 500 ug | – | in stock | |
| 3708-5mg | 5 mg | $299 | in stock |
RAPID DiO (also known as FAST DiO™ and Dilinoleyl DiO) is a cyanine dye with green fluorescence, an unsaturated analog of DiO (DiOC18(3)).
RAPID DiO is a lipophilic dye that labels cell membranes by inserting its two long hydrocarbon (C18 carbon) chains into the lipid bilayer. The dye is weakly fluorescent until incorporated into membranes. RAPID DiO diffuses laterally to stain the entire cell, allowing it to be used as an anterograde and retrograde tracer of neurons. In intact tissue, the dye does not transfer from labeled to unlabeled cells, but some transfer may occur when the membrane is disrupted, for example, after sectioning. RAPID DiO has about 50% faster migration kinetics than DiO.
RAPID DiO can be used with other tracers in dual-color studies, such as RAPID DiI.
Absorption and emission spectra of RAPID DiO
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| Appearance: | orange plasticine-like |
| Molecular weight: | 923.28 |
| Molecular formula: | C54H77F3N2O5S |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 487 |
| ε, L⋅mol−1⋅cm−1: | 166000 |
| Emission maximum, nm: | 503 |
| Fluorescence quantum yield: | 0.1 |
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