DDTT Sulfurizing Reagent

Cat. # Quantity Price Lead time
3179-1g 1 g –   in stock
3179-5g 5 g $245 in stock
3179-25g 25 g $1150 in stock
3179-50g 50 g $1800 in stock
3179-100g 100 g please inquire in stock
Found better price? Let us know and we will propose the way forward!

DDTT is a sulfurizing reagent used to prepare phosphorothioate linkages, which increase the stability and nuclease resistance of nucleic acids, especially of RNA. It is suitable for automated synthesizers employing standard phosphoramidites for a large-scale synthesis of oligonucleotides.

DDTT demonstrates faster kinetics and higher stability in solution, compared to other sulfurizing reagents such as tetraethylthiuram disulfide (TETD) and Beaucage reagent, respectively. DDTT is commonly applied as a 0.05 M or 0.1 M solution in pyridine/acetonitrile and has a lifetime of up to 6 months.

Usage

Coupling: Sulfurization step must come before capping step. The optimum sulfurizing time for DDTT Sulfurizing Reagent, 0.05 M solution in pyridine/acetonitrile: 1 min for DNA or 6 min for RNA.

Deprotection: standard conditions.

Customers also purchased with this product

sulfo-Cyanine7 carboxylic acid

sulfo-Cyanine7 carboxylic acid is a non-reactive fluorescent dye that can be used as a NIR (near-infrared) marker.

sulfo-Cyanine5.5 NHS ester

Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.

PreciseGreen dsDNA Quantification Reagent, 200×

PreciseGreen is a dye for precise fluorescence-based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.

General properties

Appearance: lemon yellow crystals
Molecular weight: 205.33
CAS number: 1192027-04-5
Molecular formula: C5H7N3S3
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Deprotection conditions: identical to protected nucleobases
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.