DDTT Sulfurizing Reagent
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
3179-1g | 1 g | – | in stock | |
3179-5g | 5 g | $245 | in stock | |
3179-25g | 25 g | $1150 | in stock | |
3179-50g | 50 g | $1800 | in stock | |
3179-100g | 100 g | please inquire | in stock |
DDTT is a sulfurizing reagent used to prepare phosphorothioate linkages, which increase the stability and nuclease resistance of nucleic acids, especially of RNA. It is suitable for automated synthesizers employing standard phosphoramidites for a large-scale synthesis of oligonucleotides.
DDTT demonstrates faster kinetics and higher stability in solution, compared to other sulfurizing reagents such as tetraethylthiuram disulfide (TETD) and Beaucage reagent, respectively. DDTT is commonly applied as a 0.05 M or 0.1 M solution in pyridine/acetonitrile and has a lifetime of up to 6 months.
Usage
Coupling: Sulfurization step must come before capping step. The optimum sulfurizing time for DDTT Sulfurizing Reagent, 0.05 M solution in pyridine/acetonitrile: 1 min for DNA or 6 min for RNA.
Deprotection: standard conditions.
Customers also purchased with this product
sulfo-Cyanine5.5 NHS ester
Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.PreciseGreen dsDNA Quantification Reagent, 200×
PreciseGreen is a dye for precise fluorescence-based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.JOE NHS ester, 5-isomer
JOE is a fluorescent dye with emission in the yellow part of the spectrum. This derivative is an activated NHS ester for conjugation with amines.General properties
Appearance: | lemon yellow crystals |
Molecular weight: | 205.33 |
CAS number: | 1192027-04-5 |
Molecular formula: | C5H7N3S3 |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | Download |
Product specifications |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | standard coupling, identical to normal nucleobases |
Deprotection conditions: | identical to protected nucleobases |