JOE NHS ester, 5-isomer

Cat. # Quantity Price Lead time
11120 1 mg $110 in stock
21120 5 mg $210 in stock
41120 25 mg $410 in stock
51120 50 mg $695 in stock
61120 100 mg $1190 in stock
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JOE is a fluorescein-derived xanthene dye containing methoxy groups and chlorine atoms. Its absorption and emission spectra are between FAM and TAMRA/ROX channels. For this reason, this fluorophore is often used for multiplex detection, including DNA sequencing.

This derivative is an activated N-hydroxysuccinimide ester used to label proteins, peptides, amino-modified oligonucleotides, and other target molecules via conjugation with amines.

Absorption and emission spectra of JOE dye

Absorption and emission spectra of JOE dye

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General properties

Appearance: orange powder
Molecular weight: 602.33
CAS number: 113394-24-4
Molecular formula: C27H17Cl2NO11
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 554
Fluorescence quantum yield: 0.61
CF260: 0.36
CF280: 0.28
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