JOE NHS ester, 5-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
11120 | 1 mg | $110 | in stock | |
21120 | 5 mg | $210 | in stock | |
41120 | 25 mg | $410 | in stock | |
51120 | 50 mg | $695 | in stock | |
61120 | 100 mg | $1190 | in stock |
JOE is a fluorescein-derived xanthene dye containing methoxy groups and chlorine atoms. Its absorption and emission spectra are between FAM and TAMRA/ROX channels. For this reason, this fluorophore is often used for multiplex detection, including DNA sequencing.
This derivative is an activated N-hydroxysuccinimide ester used to label proteins, peptides, amino-modified oligonucleotides, and other target molecules via conjugation with amines.
Absorption and emission spectra of JOE dye
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Appearance: | orange powder |
Molecular weight: | 602.33 |
CAS number: | 113394-24-4 |
Molecular formula: | C27H17Cl2NO11 |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 533 |
ε, L⋅mol−1⋅cm−1: | 75000 |
Emission maximum, nm: | 554 |
Fluorescence quantum yield: | 0.61 |
CF260: | 0.36 |
CF280: | 0.28 |
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