dsGreen® for Real-Time PCR, 100×

PCR and qPCR

Cat. #	Quantity	Price	Lead time
A1010	50 uL	–	in stock
11010	100 uL	$39	in stock
41010	1 mL	$235	in stock
71010	5 mL	$510	in stock
91010	50 mL	$2670	in stock

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dsGreen® is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.

Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:

Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot
PCR tested preparation – quality guaranteed
Low fluorescence background – high fluorescence intensity gain

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Excitation and emission spectra of dsDNA complex with dsGreen

Recommended protocol

qPCR with dsGreen® Dye

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General properties

Appearance:	light orange solution
Quality control:	NMR ¹H, HPLC-MS (95%), PCR testing
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	490
ε, L⋅mol⁻¹⋅cm⁻¹:	73000
Emission maximum, nm:	524
Fluorescence quantum yield:	0,8

Product citations

Huber, R.; Lee, J.; Borretta, L.; Tessier-Cloutier, B.; Lum, A.; Yip, S.; Horst, B.A. TERT promoter mutations in atypical melanocytic lesions: A series of seven cases with adverse melanoma-specific outcome. Human pathology, 2024, 144, 34-39. doi: 10.1016/j.humpath.2024.01.003
Mazunina, E.P.; Gushchin, V.A.; Kleymenov, D.A.; Siniavin, A.E.; Burtseva, E.I.; Shmarov, M.M.; Mukasheva, E.A.; Bykonia, E.N.; Kozlova, S.R.; Evgrafova, E.A.; Zolotar, A.N.; Shidlovskaya, E.V.; Kirillova, E.S.; Krepkaia, A.S.; Usachev, E.V.; Kuznetsova, N.A.; Ivanov, I.A.; Dmitriev, S.E.; Ivanov, R.A.; Logunov, D.Y.; Gintsburg, A.L. Trivalent mRNA vaccine-candidate against seasonal flu with cross-specific humoral immune response. Frontiers in Immunology, 2024, 15, 1381508. doi: 10.3389/fimmu.2024.1381508
Koryukov, M.A.; Oscorbin, I.P.; Novikova, L.M.; Gordukova, M.A.; Turina, I.E.; Galeeva, E.V.; Kudlay, D.A.; Filipenko, M.L. A Novel Multiplex LAMP Assay for the Detection of Respiratory Human Adenoviruses. International Journal of Molecular Sciences, 2024, 25(13), 7215. doi: 10.3390/ijms25137215
Shkodenko, L.A.; Mohamed, A.-A.; Ateiah, M.; Rubel, M.S.; Koshel, E.I. A DAMP-Based Assay for Rapid and Affordable Diagnosis of Bacterial Meningitis Agents: Haemophilus influenzae , Neisseria meningitidis , and Streptococcus pneumoniae. International Journal of Molecular Sciences, 2024, 25(15), 8282. doi: 10.3390/ijms25158282

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This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.