Cyanine3.5 NHS ester

Cyanine3.5 NHS esters Protein labeling
Cat. # Quantity Price Lead time
12020 1 mg $125 in stock
22020 5 mg $260 in stock
42020 25 mg $510 in stock
52020 50 mg $895 in stock
62020 100 mg $1490 in stock
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Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.

Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, DyLight 594.

Cyanine3.5 NHS ester, 12020
Cyanine3.5 NHS ester, 12020
Cyanine3.5 NHS ester, 12020
Structure of Cyanine3.5 NHS ester
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Cyanine3.5 NHS ester absorbance and emission spectra

Cyanine3.5 NHS ester absorbance and emission spectra

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Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

AF 343 (Coumarin)-X-azide

AF 343 (Coumarin) is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by сlick chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility and provides spatial separation between the dye and the biomolecule.

Cyanine3.5 carboxylic acid

Unactivated monofunctional Cy3.5 carboxylic acid. Can be used as a reference non-reactive dye or control.

General properties

Appearance: dark purple solid
Molecular weight: 741.62
CAS number: 2231670-85-0
Molecular formula: C42H44N3BF4O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 591
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35
CF260: 0.29
CF280: 0.22

Product citations

  1. Son, T.; Kim, M.; Choi, M.; Nam, S.H.; Yoo, A.; Lee, H.; Han, E.H.; Hong, K.S.; Park, H.S. Advancing fluorescence imaging: enhanced control of cyanine dye-doped silica nanoparticles. Journal of Nanobiotechnology, 2024, 22, 347. doi: 10.1186/s12951-024-02638-7
  2. Tang, M.; Chen, B.; Xia, H.; Pan, M.; Zhao, R.; Zhou, J.; Yin, Q.; Wan, F.; Yan, Y.; Fu, C.; Zhong, L.; Zhang, Q.; Wang, Y. pH-gated nanoparticles selectively regulate lysosomal function of tumour-associated macrophages for cancer immunotherapy. Nature Communications, 2023, 14, 5888. doi: 10.1038/s41467-023-41592-0
  3. Yin, Q.; Pan, A.; Chen, B.; Wang, Z.; Tang, M.; Yan, Y.; Wang, Y.; Xia, H.; Chen, W.; Du, H.; Chen, M.; Fu, C.; Wang, Y.; Yuan, X.; Lu, Z.; Zhang, Q.; Wang, Y. Quantitative Imaging of Intracellular Nanoparticle Exposure Enables Prediction of Nanotherapeutic Efficacy. Nat Commun, 2021, 12(1), 2385. doi: 10.1038/s41467-021-22678-z
  4. Lee, S.A.; Biteen, J.S. Spectral Reshaping of Single Dye Molecules Coupled to Single Plasmonic Nanoparticles. Journal of Physical Chemistry Letters, 2019, 10, 5764–5769. doi: 10.1021/acs.jpclett.9b02480
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Cy® is a trademark of Cytiva.
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