FAM CPG 500, 6-isomer

Cat. # Quantity Price Lead time
15760 100 mg $95 in stock
35760 500 mg $295 in stock
45760 1 g $469 in stock
55760 5 g please inquire in stock
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Controlled pore glass solid supports are used for the synthesis of 3'-labeled oligonucleotides. This solid support is intended for the synthesis of oligonucleotides bearing fluorescein (FAM) fluorescent dye on the 3'-terminus of oligonucleotide. It contains pure 6-isomer of fluorescein. The structure of the reagent is based on a chiral, enantiomerically pure scaffold of hydroxyprolinol.

The solid support ensures optimal yield of oligonucleotides up to 60mer. For longer oligos, CPG 1000 should be used. The reagent is compatible with standard ammonia cleavage and deblocking conditions.

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General properties

Appearance: off white beads
Quality control: NMR 1H and loading measurement, functional testing in oligo synthesis.
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 65±15
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases

Product citations

  1. Zharkov, T.D.; Mironova, E.M.; Markov, O.V.; Zhukov, S.A.; Khodyreva, S.N.; Kupryushkin, M.S. Fork- and Comb-like Lipophilic Structures: Different Chemical Approaches to the Synthesis of Oligonucleotides with Multiple Dodecyl Residues. International Journal of Molecular Sciences, 2023, 24(19), 14637. doi: 10.3390/ijms241914637
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