Alkyne dT phosphoramidite
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1897-100mg | 100 mg |
$330
$195
|
in stock | |
| 1897-1g | 1 g |
$2590
$1250
|
in stock | |
| 1897-5g | 5 g |
$9900
$2850
|
in stock |
This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to deoxyuridine fragment, and it does not interfere with the hybridization to the complementary strand. This allows to prepare modified oligonucleotide probes bearing fluorescent dyes, quenchers, and other fragments attached to the middle of the chain, by virtue of copper catalyzed Click chemistry reaction with various azides.
This phosphoramidite is solid, so it is easy to dispense and handle. It is compatible with the standard deblocking conditions.
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Copper(II)-TВTA catalytic buffer, 1.5x
Ready-to-use catalytic buffer containing сopper(II) and TBTA ligand. It is suitable for click chemistry modification of nucleic acids and small molecules.General properties
| Appearance: | off white solid |
| Mass spec M+ increment: | 437.1 |
| Molecular weight: | 877.96 |
| Molecular formula: | C48H56N5O9P |
| Solubility: | good in acetonitrile, DCM |
| Quality control: | NMR 1H, NMR 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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