JOE phosphoramidite, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12160 | 100 mg | – | in stock | |
| D2160 | 250 mg | $165 | in stock | |
| F2160 | 1 g |
$575
|
in stock | |
| G2160 | 5 g |
$2230
|
in stock | |
| H2160 | 10 g |
$4180
|
in stock | |
| K2160 | 20 g | please inquire | in stock |
JOE phosphoramidite for oligonucleotide synthesis, pure 6-isomer (6-JOE).
Fluorescent dye JOE is a fluorescein derivative containing two chlorine atoms and two methoxy groups. Its absorption and emission maxima are at 533 nm and 554 nm, respectively. By its spectral characteristics JOE is found in between FAM and TAMRA/ROX; therefore, this fluorophor is commonly used for multiplex detection, including that during DNA sequencing.
Our catalog also contains JOE phosphoramidite, 5-isomer. We compared qPCR probes containing different JOE isomers (5-JOE and 6-JOE) and did not find any significant differences between them.
Usage
Coupling: 6 minutes
Deprotection: standard conditions using ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °С removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).
Absorption and emission spectra of JOE
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General properties
| Appearance: | white solid |
| Molecular weight: | 972.88 |
| Molecular formula: | C48H60N3Cl2O12P |
| Solubility: | Good solubility in acetonitrile and DCM |
| Quality control: | NMR 1H and 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 554 |
| Fluorescence quantum yield: | 0.61 |
| CF260: | 0.36 |
| CF280: | 0.28 |
Oligo synthesis details
| Diluent: | acetonitrile |
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