BDP® FL tetrazine
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
114E0 | 1 mg | $110 | in stock | |
214E0 | 5 mg | $290 | in stock | |
414E0 | 25 mg | $690 | in stock | |
514E0 | 50 mg | $1270 | in stock | |
614E0 | 100 mg | $1990 | in stock |
Inverse electron demand Diels-Alder reaction (IEDDA) with tetrazines is a promising tool for the conjugation of biomolecules. The reaction takes place between tetrazine as an electron acceptor heterodiene, and a strained dienophile, such as trans-cyclooctene, cyclopropene, or some cyclooctynes.
Methyltetrazine provides greater stability in buffers than unsubstituted tetrazine. The rate of its reaction with cycloalkenes still beats almost all other conjugation reaction rates by a factor of magnitudes.
BDP FL is a bright dye for fluorescein (FAM) channel. Using BDP FL tetrazine, the dye can be conjugated with molecules bearing strained olefins.
Absorption and emission spectra of BDP FL
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Appearance: | red crystalline solid |
Mass spec M+ increment: | 447.2 |
Molecular weight: | 475.3 |
CAS number: | 2042193-77-9 |
Molecular formula: | C24H24N7BF2O |
Solubility: | good in DCM, DMSO, DMF |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 503 |
ε, L⋅mol−1⋅cm−1: | 92000 |
Emission maximum, nm: | 509 |
Fluorescence quantum yield: | 0.97 |
CF260: | 0.015 |
CF280: | 0.027 |