BDP® FL tetrazine

BDP® FL Tetrazines Copper-free click chemistry

Cat. #	Quantity	Price	Lead time
114E0	1 mg	$110	in stock
214E0	5 mg	$290	in stock
414E0	25 mg	$690	in stock
514E0	50 mg	$1270	in stock
614E0	100 mg	$1990	in stock

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Inverse electron demand Diels-Alder reaction (IEDDA) with tetrazines is a promising tool for the conjugation of biomolecules. The reaction takes place between tetrazine as an electron acceptor heterodiene, and a strained dienophile, such as trans-cyclooctene, cyclopropene, or some cyclooctynes.

Methyltetrazine provides greater stability in buffers than unsubstituted tetrazine. The rate of its reaction with cycloalkenes still beats almost all other conjugation reaction rates by a factor of magnitudes.

BDP FL is a bright dye for fluorescein (FAM) channel. Using BDP FL tetrazine, the dye can be conjugated with molecules bearing strained olefins.

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Absorption and emission spectra of BDP FL

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AF 488 DBCO

Dibenzocyclooctyne (DBCO, ADIBO) containing the green fluorophore AF 488 for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples. Pure 5-isomer.

BDP® FL DBCO

BDP FL DBCO is a dye cyclooctyne for copper-free click chemistry reactions with azides. Its absorption and emission wavelengths correspond to those of fluorescein (FAM), but BDP FL is significantly more photostable.

General properties

Appearance:	red crystalline solid
Mass spec M+ increment:	447.2
Molecular weight:	475.3
CAS number:	2042193-77-9
Molecular formula:	C₂₄H₂₄N₇BF₂O
Solubility:	good in DCM, DMSO, DMF
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	503
ε, L⋅mol⁻¹⋅cm⁻¹:	92000
Emission maximum, nm:	509
Fluorescence quantum yield:	0.97
CF₂₆₀:	0.015
CF₂₈₀:	0.027

BDP® is a trademark of Lumiprobe

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.