Copper(II)-THPTA catalytic buffer, 1.5x
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| B5150 | 1 mL | $30 | in stock | |
| K5150 | 10 mL | $120 | in stock | |
| S5150 | 50 mL |
$490
|
in stock |
Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5x buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.
Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine
Recommended protocol
Calculator
Customers also purchased with this product
TBTA ligand
TBTA is one of the most widely used water-insoluble ligands for copper-catalyzed azide-alkyne cycloadditions (CuAAC).Alkyne-PEG4-maleimide
Water soluble, heterobifunctional hydrophilic linker with a length of 14 Å, containing maleimide for thiol labeling, and alkyne for click chemistry. An ideal reagent to attach an alkyne moiety to a protein.Alkyne CPG 1000A
Controlled pore glass solid support for DNA/RNA synthesis with alkyne modification (pore size 1000 Å) for synthesizing long-modified oligonucleotides with 3'-alkyne modification.General properties
| Storage conditions: | Storage: 12 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks. |
| MSDS: | Download |
| Product specifications |
Your cart is empty
$ 
