Copper(II)-THPTA catalytic buffer, 1.5x
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
B5150 | 1 mL | $30 | in stock | |
K5150 | 10 mL | $120 | in stock | |
S5150 | 50 mL | $490 | in stock |
Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5x buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.
Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine
Recommended protocol
Calculator
Customers also purchased with this product
Hexynoic acid NHS ester
Hexynoic acid NHS ester for the labeling of biomolecules with alkyne group for copper-catalyzed click chemistry.AF 568 azide
Azide derivative of water-soluble AF 568 dye for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects. Pure 6-isomer.LumiTracker® Mito JC-1
The cationic carbocyanine dye that is widely used as an indicator of mitochondrial membrane potential to study apoptosis and monitor the health of mitochondria.General properties
Storage conditions: | Storage: 12 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks. |
MSDS: | Download |
Product specifications |