TAMRA CPG 500, 5-isomer

TAMRA (TMR, Tetramethylrhodamine) CPG modifier solid supports
Cat. # Quantity Price Lead time
2792-100mg 100 mg $95 in stock
2792-500mg 500 mg $295 in stock
2792-1g 1 g $469 in stock
2792-5g 5 g please inquire in stock
Bulk inquiry
Found better price? Let us know and we will propose the way forward!

Controlled pore glass solid support with TAMRA for the synthesis of 3'-labeled oligonucleotides.

TAMRA CPG supports allow for the introduction of a reporter/quencher to be used in probe-based assays, like TaqMan probes for real-time PCR quantification and FRET experiments, which require that oligonucleotides be doubly labeled.

The proposed solid support 500 Å provides an optimal yield of oligonucleotides up to 50 mer. The TAMRA dye is not stable in the presence of ammonium, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.

Usage

Coupling: standard time depending on the first monomer.

Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 h at 60 °C, then cool down to room temperature.

TAMRA CPG 500, 5-isomer, 2792-1g
TAMRA CPG 500, 5-Isomer, 2792-1g
TAMRA CPG 500, 5-isomer, 2792-1g
Structure of TAMRA CPG 500, 5-isomer
Download in
ChemDraw format

Customers also purchased with this product

DusQ 1 dT phosphoramidite

A phosphoramidite used for DusQ 1 dT labelling of oligonucleotides.
Add this product to your cart and
get free express delivery

Cyanine5.5 phosphoramidite

Amidite for the synthesis of oligonucleotides and qPCR probes bearing 5'-terminal dye Cyanine5.5. Useful dye for multiplex qPCR.
Add this product to your cart and
get free express delivery

TR azide, 5-isomer

Azide derivative of red fluorescent dye TR for сlick сhemistry conjugation. Pure 5-isomer.

General properties

Appearance: deep pink beads
Quality control: NMR 1H and loading measurement, functional testing in oligo synthesis.
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 541
ε, L⋅mol−1⋅cm−1: 84000
Emission maximum, nm: 567
Fluorescence quantum yield: 0.1
CF260: 0.32
CF280: 0.19

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−70

Product citations

  1. Bauer, I.; Ilina, E.; Zharkov, T.; Grigorieva, E.; Chinak, O.; Kupryushkin, M.; Golyshev, V.; Mitin, D.; Chubarov, A.; Khodyreva, S.; Dmitrienko, E. Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 2023, 15(12), 2779. doi: 10.3390/pharmaceutics15122779
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.