TAMRA CPG 500, 5-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
2792-100mg | 100 mg | $95 | in stock | |
2792-500mg | 500 mg | $295 | in stock | |
2792-1g | 1 g |
$469
|
in stock | |
2792-5g | 5 g | please inquire | in stock |
Controlled pore glass solid support with TAMRA for the synthesis of 3'-labeled oligonucleotides.
TAMRA CPG supports allow for the introduction of a reporter/quencher to be used in probe-based assays, like TaqMan probes for real-time PCR quantification and FRET experiments, which require that oligonucleotides be doubly labeled.
The proposed solid support 500 Å provides an optimal yield of oligonucleotides up to 50 mer. The TAMRA dye is not stable in the presence of ammonium, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
Usage
Coupling: standard time depending on the first monomer.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 h at 60 °C, then cool down to room temperature.
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Alkyne CPG modifier 500
Solid support for the synthesis of 3'-modified oligonucleotides with alkyne group for Click Chemistry.Universal CPG type II, 1000A
This universal support is intended for automated synthesis of oligonucleotides to accelerate the dephosphorylation process during deblocking. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides up to 120 bases.FAM NHS ester, 6-isomer
Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.General properties
Appearance: | deep pink beads |
Quality control: | NMR 1H and loading measurement, functional testing in oligo synthesis. |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 541 |
ε, L⋅mol−1⋅cm−1: | 84000 |
Emission maximum, nm: | 567 |
Fluorescence quantum yield: | 0.1 |
CF260: | 0.32 |
CF280: | 0.19 |
Oligo synthesis details
Pore size, Å: | 500 |
Typical loading, umol/g: | 50−70 |
Product citations
- Bauer, I.; Ilina, E.; Zharkov, T.; Grigorieva, E.; Chinak, O.; Kupryushkin, M.; Golyshev, V.; Mitin, D.; Chubarov, A.; Khodyreva, S.; Dmitrienko, E. Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 2023, 15(12), 2779. doi: 10.3390/pharmaceutics15122779
