AF 555 azide

Azides AF 555 Click chemistry

Cat. #	Quantity	Price	Lead time
SK830	250 ug	–	in stock
1K830	1 mg	$140	in stock
2K830	5 mg	$450	in stock
4K830	25 mg	$1460	in stock
5K830	50 mg	$1890	in stock
6K830	100 mg	$2640	in stock

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AF 555 dye azide for сlick сhemistry conjugation with terminal alkynes via a copper-catalyzed click reaction or strained cyclooctynes via a copper-free click reaction.

AF 555 is a hydrophilic fluorophore with high fluorescence quantum yield and high photostability, an alternative to tetramethylrhodamine (TAMRA, TMR) or Cyanine3 dyes. The dye is useful for many applications, including cytometry and microscopy.

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Absorption and emission spectra of AF 555

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Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes

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AF 343 (Coumarin)-X-NHS ester

AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.

General properties

Appearance:	dark red solid
Molecular weight:	1043.40
Molecular formula:	C₃₈H₄₉K₃N₆O₁₃S₄
Solubility:	good in water, DMF, DMSO
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	552
ε, L⋅mol⁻¹⋅cm⁻¹:	152000
Emission maximum, nm:	566
Fluorescence quantum yield:	0.14

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.