TODi-3, far-red fluorescent nucleic acid stain

Cell viability and apoptosis Nuclei Flow cytometry Microscopy
Cat. # Quantity Price Lead time
A2610 50 uL, 5 mM/DMSO $85 in stock
22610 200 uL, 5 mM/DMSO $320 in stock
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TODi-3 (Thiazole Red Homodimer, also known as TOTO®-3) is a far-red fluorescent carbocyanine dimeric dye. TODi-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a significant fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make TODi-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments. TODi-3 is non-cytotoxic and may be used for long-term monitoring of cell viability and dead cell detection in culture. The long-wavelength fluorescence of TO-TAP-3 is well separated from green and red fluorophores, which makes it ideal for multicolor fluorescence labeling experiments.

TODi-3, far-red fluorescent nucleic acid stain, 22610
TODi-3, far-red fluorescent nucleic acid stain, 22610
TODi-3, far-red fluorescent nucleic acid stain, 22610
Structure of TODi-3
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Absorption and emission spectra of TODi-3 (DNA–dye complex)

Absorption and emission spectra of TODi-3 (DNA–dye complex)

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General properties

Appearance: blue solution
Molecular weight: 1354.87
CAS number: 166196-17-4
Molecular formula: C53H62I4N6S2
IUPAC name: Quinolinium, 1,1′-[1,3-propanediylbis[(dimethyliminio)-3,1-propanediyl]]bis[4-[3-(3-methyl-2(3H)-benzothiazolylidene)-1-propenyl]-, tetraiodide
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 644
Emission maximum, nm: 662
TOTO® is the trademark of Invitrogen.
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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