AF 532 NHS ester

NHS esters AF 532 Protein labeling

Cat. #	Quantity	Price	Lead time
1C820	1 mg	$140	in stock
2C820	5 mg	$450	in stock
4C820	25 mg	$1460	in stock
5C820	50 mg	$1890	in stock
6C820	100 mg	$2640	in stock

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Activated NHS-ester of AF 532 dye. This NHS ester is an amine-reactive dye for labeling of amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

AF 532 is a bright, photostable, and hydrophilic fluorophore emitting in the yellow-green channel, an alternative to HEX, JOE, and SIMA. The dye is useful in super-resolution microscopy, particularly in stochastic optical reconstruction microscopy (STORM), as an activator in nSTORM and a reporter in dSTORM.

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Absorption and emission spectra of AF 532

General properties

Appearance:	dark red powder
Molecular weight:	824.96
Molecular formula:	C₄₀H₄₈N₄O₁₁S₂
Solubility:	water, DMSO, DMF
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	524
ε, L⋅mol⁻¹⋅cm⁻¹:	80500
Emission maximum, nm:	547
Fluorescence quantum yield:	0.6
CF₂₆₀:	0.17
CF₂₈₀:	0.11

Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.