sulfo-Cyanine5.5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A7330 | 1 mg | $110 | in stock | |
| B7330 | 5 mg | $325 | in stock | |
| C7330 | 10 mg |
$470
|
in stock | |
| D7330 | 25 mg |
$850
|
in stock | |
| E7330 | 50 mg |
$1490
|
in stock | |
| F7330 | 100 mg |
$2590
|
in stock |
sulfo-Cyanine5.5 is a water-soluble, hydrophilic fluorophore with emission in the far-red area of the spectrum, an analog of Cy5.5®. Like other cyanine dyes, sulfo-Cyanine5.5 has an outstanding molar extinction coefficient, giving rise to its bright fluorescence. The molecule contains four sulfo groups that provide hydrophilicity and negative charge to the fluorophore — this minimizes non-specific binding.
The azide group of Sulfo-Cyanine5.5 azide can be conjugated with terminal alkynes in the presence of copper(I) catalyst or with cycloalkynes in copper-free strain-promoted reaction.
The reagent possesses high hydrophilicity and is recommended for labeling biomolecules in an aqueous environment.
Absorption and emission spectra of sulfo-Cyanine5.5
Recommended protocol
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sulfo-Cyanine3 azide
Water-soluble sulfo-Cyanine3 dye azide for click chemistry labeling of sensitive molecules and intact biological objects.BDP® 558/568 NHS ester
BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.TAMRA hydrazide, 6-isomer
6-TAMRA azide is a carbonyl reactive hydrazide derivative of tetramethylrhodamine (TAMRA) fluorophore.General properties
| Appearance: | dark colored solid |
| Mass spec M+ increment: | 984.2 |
| Molecular weight: | 1099.41 |
| CAS number: | 2382994-65-0 |
| Molecular formula: | C43H45K3N6O13S4 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 673 |
| ε, L⋅mol−1⋅cm−1: | 211000 |
| Emission maximum, nm: | 691 |
| Fluorescence quantum yield: | 0.21 |
| CF260: | 0.09 |
| CF280: | 0.11 |
Product citations
- Gleixner, J.; Kopanchuk, S.; Grätz, L.; Tahk, M.-J.; Laasfeld, T.; Veikšina, S.; Höring, C.; Gattor, A. O.; Humphrys, L. J.; Müller, C.; Archipowa, N.; Köckenberger, J.; Heinrich, M. R.; Kutta, R. J.; Rinken, A.; Keller, M. Illuminating Neuropeptide Y Y4 Receptor Binding: Fluorescent Cyclic Peptides with Subnanomolar Binding Affinity as Novel Molecular Tools. ACS Pharmacol. Transl. Sci., 2024, 7(4), 1142–1168. doi: 10.1021/acsptsci.4c00013
- Sajeesh, S.; Camardo, A.; Dahal, S.; Ramamurthi, A. Surface-Functionalized Stem Cell-Derived Extracellular Vesicles for Vascular Elastic Matrix Regenerative Repair. Molecular Pharmaceutics, 2023, 20(6), 2801-2813. doi: 10.1021/acs.molpharmaceut.2c00769
- Erel-Akbaba, G.; Carvalho, L.A.; Tian, T.; Zinter, M.; Akbaba, H.; Obeid, P.J.; Chiocca, E.A.; Weissleder, R.; Kantarci, A.G.; Tannous, B.A. Radiation-Induced Targeted Nanoparticle-Based Gene Delivery for Brain Tumor Therapy. ACS Nano, 2019, 13(4), 4028–4040. doi: 10.1021/acsnano.8b08177
- Gu, W.; Bobrin, V.A.; Chen, S.-P.R.; Wang, Z.; Schoning, J.P.; Gu, Y.; Chen, W.; Chen, M.; Jia, Z.; Monteiro, M.J. Biodistribution of PNIPAM-Coated Nanostructures Synthesized by the TDMT Method. Biomacromolecules, 2019, 20(2), 625–634. doi: 10.1021/acs.biomac.8b01196
Cy® is a trademark of GE Healthcare.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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