sulfo-Cyanine5.5 azide
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
A7330 | 1 mg | $110 | in stock | |
B7330 | 5 mg | $325 | in stock | |
C7330 | 10 mg | $470 | in stock | |
D7330 | 25 mg | $850 | in stock | |
E7330 | 50 mg | $1490 | in stock | |
F7330 | 100 mg | $2590 | in stock |
sulfo-Cyanine5.5 is a water-soluble, hydrophilic fluorophore with emission in the far-red area of the spectrum, an analog of Cy5.5®. Like other cyanine dyes, sulfo-Cyanine5.5 has an outstanding molar extinction coefficient, giving rise to its bright fluorescence. The molecule contains four sulfo groups that provide hydrophilicity and negative charge to the fluorophore — this minimizes non-specific binding.
The azide group of Sulfo-Cyanine5.5 azide can be conjugated with terminal alkynes in the presence of copper(I) catalyst or with cycloalkynes in copper-free strain-promoted reaction.
The reagent possesses high hydrophilicity and is recommended for labeling biomolecules in an aqueous environment.
Absorption and emission spectra of sulfo-Cyanine5.5
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FAM azide, 6-isomer
FAM (fluorescein) azide, 6-isomer is a commonly used fluorescent dye azide for сlick сhemistry.Azide-PEG6-NHS ester
A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).Copper(II)-TBTA complex
A catalyst for click reaction. A stable copper(II) complex which must be converted to monovalent copper(I) in situ using reducing agents. The complex can be used to prepare a reaction buffer of any composition.General properties
Appearance: | dark colored solid |
Mass spec M+ increment: | 984.2 |
Molecular weight: | 1099.41 |
CAS number: | 2382994-65-0 |
Molecular formula: | C43H45K3N6O13S4 |
Solubility: | good in water, DMF, DMSO |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 673 |
ε, L⋅mol−1⋅cm−1: | 211000 |
Emission maximum, nm: | 691 |
Fluorescence quantum yield: | 0.21 |
CF260: | 0.09 |
CF280: | 0.11 |
Product citations
- Gleixner, J.; Kopanchuk, S.; Grätz, L.; Tahk, M.-J.; Laasfeld, T.; Veikšina, S.; Höring, C.; Gattor, A. O.; Humphrys, L. J.; Müller, C.; Archipowa, N.; Köckenberger, J.; Heinrich, M. R.; Kutta, R. J.; Rinken, A.; Keller, M. Illuminating Neuropeptide Y Y4 Receptor Binding: Fluorescent Cyclic Peptides with Subnanomolar Binding Affinity as Novel Molecular Tools. ACS Pharmacol. Transl. Sci., 2024, 7(4), 1142–1168. doi: 10.1021/acsptsci.4c00013
- Sajeesh, S.; Camardo, A.; Dahal, S.; Ramamurthi, A. Surface-Functionalized Stem Cell-Derived Extracellular Vesicles for Vascular Elastic Matrix Regenerative Repair. Molecular Pharmaceutics, 2023, 20(6), 2801-2813. doi: 10.1021/acs.molpharmaceut.2c00769
- Erel-Akbaba, G.; Carvalho, L.A.; Tian, T.; Zinter, M.; Akbaba, H.; Obeid, P.J.; Chiocca, E.A.; Weissleder, R.; Kantarci, A.G.; Tannous, B.A. Radiation-Induced Targeted Nanoparticle-Based Gene Delivery for Brain Tumor Therapy. ACS Nano, 2019, 13(4), 4028–4040. doi: 10.1021/acsnano.8b08177
- Gu, W.; Bobrin, V.A.; Chen, S.-P.R.; Wang, Z.; Schoning, J.P.; Gu, Y.; Chen, W.; Chen, M.; Jia, Z.; Monteiro, M.J. Biodistribution of PNIPAM-Coated Nanostructures Synthesized by the TDMT Method. Biomacromolecules, 2019, 20(2), 625–634. doi: 10.1021/acs.biomac.8b01196