sulfo-Cyanine3 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11320 | 1 mg | $91 | in stock | |
| 21320 | 5 mg | $119 | in stock | |
| 41320 | 25 mg | $395 | in stock | |
| 51320 | 50 mg |
$729
|
in stock | |
| 61320 | 100 mg |
$1290
|
in stock |
Water soluble, amino-reactive sulfo-Cyanine3 NHS ester that efficiently labels proteins and peptides in purely aqueous solution without needing organic co-solvent. Ideal for proteins with low solubility and proteins prone to denaturation.
This is the sulfonated, hydrophilic, and water-soluble dye. Non-sulfonated Cyanine3 NHS ester is also available.
This product is an analog of Cy3® NHS ester. sulfo-Cyanine3 NHS ester replaces Cy3® and DyLight 549 for all applications.
sulfo-Cyanine3 absorbance and emission spectra
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| Appearance: | dark red crystals |
| Molecular weight: | 751.91 |
| CAS number: | 1424150-38-8 (sodium salt); 1424433-17-9, 1518643-34-9 (inner salt) |
| Molecular formula: | C34H38N3KO10S2 |
| IUPAC name: | 3H-Indolium, 2-[3-(1,3-dihydro-1,3,3-trimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3,3-dimethyl-5-sulfo-, inner salt, sodium salt |
| Solubility: | soluble in water (0.62 M = 47 g/L), in polar organic solvents (DMF, DMSO) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
Product citations
- Yang, B.; Picchetti, P.; Wang, Y.; Wang, W.; Seeger, C.; Bozov, K.; Malik, S.; Mallach, D.; Schäfer, A.H.; Ibrahim, M.; Hirtz, M.; Powell, A.K. Patterned immobilization of polyoxometalate-loaded mesoporous silica particles via amine-ene Michael additions on alkene functionalized surfaces. Scientific Reports, 2024, 14, 1249. doi: 10.1038/s41598-023-50846-2
- Lebedeva, N.A.; Anarbaev, R.O.; Maltseva, E.A.; Sukhanova, M.V.; Rechkunova, N.I.; Lavrik, O.I. DNA Repair Protein XRCC1 Stimulates Activity of DNA Polymerase λ under Conditions of Microphase Separation. International Journal of Molecular Sciences, 2024, 25(13), 6927. doi: 10.3390/ijms25136927
- Takahashi, S.; Homma, K. The molecular principles underlying diverse functions of the SLC26 family of proteins. The Journal of Biological Chemistry, 2024, 300(5), 107261. doi: 10.1016/j.jbc.2024.107261
- Zhao, J.; Elgeti, M.; O’Brien, E.S.; Sár, C.P.; EI Daibani, A.; Heng, J.; Sun, X.; White, E.; Che, T.; Hubbell, W.L.; Kobilka, B.K.; Chen, C. Ligand efficacy modulates conformational dynamics of the µ-opioid receptor. Nature, 2024, 629(8011), 474-480. doi: 10.1038/s41586-024-07295-2
Cy® is a trademark of Cytiva.
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