sulfo-Cyanine3 NHS ester

NHS esters sulfo-Cyanine3 Protein labeling

Cat. #	Quantity	Price	Lead time
11320	1 mg	$91	in stock
21320	5 mg	$119	in stock
41320	25 mg	$395	in stock
51320	50 mg	$729	in stock
61320	100 mg	$1290	in stock

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Water soluble, amino-reactive sulfo-Cyanine3 NHS ester that efficiently labels proteins and peptides in purely aqueous solution without needing organic co-solvent. Ideal for proteins with low solubility and proteins prone to denaturation.

This is the sulfonated, hydrophilic, and water-soluble dye. Non-sulfonated Cyanine3 NHS ester is also available.

This product is an analog of Cy3® NHS ester. sulfo-Cyanine3 NHS ester replaces Cy3® and DyLight 549 for all applications.

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sulfo-Cyanine3 absorbance and emission spectra

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General properties

Appearance:	dark red crystals
Molecular weight:	751.91
CAS number:	1424150-38-8 (sodium salt); 1424433-17-9, 1518643-34-9 (inner salt)
Molecular formula:	C₃₄H₃₈N₃KO₁₀S₂
IUPAC name:	3H-Indolium, 2-[3-(1,3-dihydro-1,3,3-trimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3,3-dimethyl-5-sulfo-, inner salt, sodium salt
Solubility:	soluble in water (0.62 M = 47 g/L), in polar organic solvents (DMF, DMSO)
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	548
ε, L⋅mol⁻¹⋅cm⁻¹:	162000
Emission maximum, nm:	563
Fluorescence quantum yield:	0.1
CF₂₆₀:	0.03
CF₂₈₀:	0.06

Product citations

Yang, B.; Picchetti, P.; Wang, Y.; Wang, W.; Seeger, C.; Bozov, K.; Malik, S.; Mallach, D.; Schäfer, A.H.; Ibrahim, M.; Hirtz, M.; Powell, A.K. Patterned immobilization of polyoxometalate-loaded mesoporous silica particles via amine-ene Michael additions on alkene functionalized surfaces. Scientific Reports, 2024, 14, 1249. doi: 10.1038/s41598-023-50846-2
Zhao, J.; Elgeti, M.; O’Brien, E.S.; Sár, C.P.; EI Daibani, A.; Heng, J.; Sun, X.; White, E.; Che, T.; Hubbell, W.L.; Kobilka, B.K.; Chen, C. Ligand efficacy modulates conformational dynamics of the µ-opioid receptor. Nature, 2024, 629(8011), 474-480. doi: 10.1038/s41586-024-07295-2
Lebedeva, N.A.; Anarbaev, R.O.; Maltseva, E.A.; Sukhanova, M.V.; Rechkunova, N.I.; Lavrik, O.I. DNA Repair Protein XRCC1 Stimulates Activity of DNA Polymerase λ under Conditions of Microphase Separation. International Journal of Molecular Sciences, 2024, 25(13), 6927. doi: 10.3390/ijms25136927
Peña-Díaz, S.; Chao, J.D.; Rens, C.; Haghdadi, H.; Zheng, X.; Flanagan, K.; Ko, M.; Shapira, T.; Richter, A.; Maestre-Batlle, D.; Canseco, J.O.; Gutierrez, M.G.; Duc, K.D.; Pelech, S.; Av-Gay, Y. Glycogen synthase kinase 3 inhibition controls Mycobacterium tuberculosis infection. iScience, 2024, 27(8), 110555. doi: 10.1016/j.isci.2024.110555

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Cy® is a trademark of Cytiva.

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.