ATT 647N NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1E920 | 1 mg |
$199
|
in stock | |
| 2E920 | 5 mg |
$746
|
in stock |
ATT 647N NHS ester is an amine-reactive dye for labeling various amine-containing molecules in an aqueous phase without using any organic co-solvent. This product is beneficial for the labeling of peptides and proteins that denature in the presence of organic co-solvents, as well as for proteins with low solubility.
ATT 647N is a rhodamine-based far-red fluorophore with strong molar absorption, high fluorescence quantum yield, and excellent thermal and photostability. ATT 647N fluorescence is independent of pH in the range of 2 to 11, which supports its application under diverse experimental conditions.
Unlike cyanine dyes, ATT 647N exhibits enhanced resistance to atmospheric ozone degradation, making it highly suitable for microarray and other high-precision applications such as single-molecule detection, super-resolution microscopy techniques (e.g., SIM and STED), flow cytometry (FACS), and fluorescence in situ hybridization (FISH).
Absorption and emission spectra of ATT 647N NHS ester
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General properties
| Appearance: | blue powder |
| Molecular weight: | 779.42 |
| CAS number: | 1199940-27-6 |
| Molecular formula: | C46H55ClN4O5 |
| Solubility: | DMSO, DCM, DMF, acetonitrile |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 644 |
| ε, L⋅mol−1⋅cm−1: | 105000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.68 |
| CF260: | 0.08 |
| CF280: | 0.05 |
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