ATT 647N NHS ester

NHS esters ATT 647N

Cat. #	Quantity	Price	Lead time	Buy this product
1E920	1 mg	$199	in stock
2E920	5 mg	$746	in stock

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ATT 647N NHS ester is an amine-reactive dye for labeling various amine-containing molecules in an aqueous phase without using any organic co-solvent. This product is beneficial for the labeling of peptides and proteins that denature in the presence of organic co-solvents, as well as for proteins with low solubility.

ATT 647N is a rhodamine-based far-red fluorophore with strong molar absorption, high fluorescence quantum yield, and excellent thermal and photostability. ATT 647N fluorescence is independent of pH in the range of 2 to 11, which supports its application under diverse experimental conditions.

Unlike cyanine dyes, ATT 647N exhibits enhanced resistance to atmospheric ozone degradation, making it highly suitable for microarray and other high-precision applications such as single-molecule detection, super-resolution microscopy techniques (e.g., SIM and STED), flow cytometry (FACS), and fluorescence in situ hybridization (FISH).

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Absorption and emission spectra of ATT 647N NHS ester

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NHS Ester Labeling of Biomolecules Containing Primary Amino Groups

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General properties

Appearance:	blue powder
Molecular weight:	779.42
CAS number:	1199940-27-6
Molecular formula:	C₄₆H₅₅ClN₄O₅
Solubility:	DMSO, DCM, DMF, acetonitrile
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	644
ε, L⋅mol⁻¹⋅cm⁻¹:	105000
Emission maximum, nm:	662
Fluorescence quantum yield:	0.68
CF₂₆₀:	0.08
CF₂₈₀:	0.05

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.