DAF-FM (4-amino-5-methylamino-2′,7′-difluorofluorescein)
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
SB100 | 50 ug | $22 | in stock | |
1B100 | 1 mg | $365 | 12 days |
DAF-FM (4-amino-5-methylamino-2′,7′-difluorofluorescein) is a cell-impermeant, fluorescent probe for detecting and quantifying low concentrations of nitric oxide (NO). DAF-FM does not need to be activated by cytosolic enzymes and is suitable to detect NO in extracellular matrix.
The fluorescence quantum yield of DAF-FM is ~0.005, but it increases about 160-fold to ~0.81 after reacting with NO and forming a fluorescent benzotriazole (excitation/emission maxima at 495/515 nm) .
The NO detection limit of DAF-FM (~3 nM) is more sensitive than that of DAF-2 (~5 nM). The fluorescence of the NO adduct of DAF-FM is independent of pH above pH 5.5. Moreover, the NO adduct of DAF-FM demonstrates a significantly enhanced photostability compared to that of DAF-2, ensuring reliable results and additional time for imaging.
DAF-FM should be dissolved in DMSO and then used to prepare a working solution. Buffers containing bovine serum albumin (BSA) or phenol red can affect the fluorescence and should be used cautiously.
The cell-permeant version of DAF FM — DAF-FM DA is also available.
Customers also purchased with this product
DAF-FM diacetate (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate)
Cell-permeant, fluorescent probe for detecting and quantifying low concentrations of nitric oxide (NO).6-Carboxy-H2DCFDA (6-carboxy-2',7'-dichlorodihydrofluorescein)
A reagent for detecting reactive oxygen species (ROS) in living cells. This carboxylated H2DCFDA analog has two additional negative charges that impede its leakage out of the cell.General properties
Appearance: | yellow to brown solid |
Molecular weight: | 412.35 |
CAS number: | 254109-20-1 |
Molecular formula: | C21H14F2N2O5 |
Solubility: | good in methanol, DMSO, DMF and water; limited in water; poor in acetonitrile and methylene chloride |
Quality control: | NMR 1H and HPLC-MS (90+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | Download |
Product specifications |