VU (5-Vinyl-uridine)
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3517-10mg | 10 mg | $125 | in stock | |
| 3517-50mg | 50 mg |
$449
|
in stock |
5-Vinyl-uridine (VU) is a uridine derivative with a terminal alkene group that can be used for monitoring and investigating RNA transcription in cells instead of 5-ethynyl-uridine (EU).
VU is readily taken up by living cells and incorporated by RNA polymerases into de novo RNA instead of endogenous uridine, but not into DNA [1]. VU-labeled nascent cellular RNA can be detected quickly and with high sensitivity via inverse electron-demand Diels-Alder cycloaddition reaction (IEDDA) between the vinyl group and fluorescently or biotin-labeled tetrazines.
Labeled RNA can be detected with different cell transcriptional levels estimation methods, e.g., fluorescent microscopy or flow cytometry.
[1] Liu H.S. et al. A Nucleoside Derivative 5-Vinyluridine (VrU) for Imaging RNA in Cells and Animals. Bioconjug.Chem. 2019. 30(11). 2958-2966.
Customers also purchased with this product
sulfo-Cyanine5.5 tetrazine
Tetrazine derivative of water-soluble far-red emitting fluorescent dye sulfo-Cyanine5.5 for TCO ligation with trans-cyclooctenes and other strained olefins.6-Carboxy-H2DCFDA (6-carboxy-2',7'-dichlorodihydrofluorescein)
A reagent for detecting reactive oxygen species (ROS) in living cells. This carboxylated H2DCFDA analog has two additional negative charges that impede its leakage out of the cell.MitoCLox, mitochondrial lipid peroxidation probe
MitoCLox is a mitochondria-targeted fluorescence probe for tracing cardiolipin oxidation in vivo.General properties
| Appearance: | white solid |
| Molecular weight: | 270.24 |
| CAS number: | 55520-64-4 |
| Molecular formula: | C11H14N2O6 |
| Solubility: | DMSO, methanol |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Your cart is empty
$ 
