Products
Cell biology reagents
ROS indicators
DAF-FM diacetate (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate)

DAF-FM diacetate (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate)

Microscopy Flow cytometry ROS sensing

Cat. #	Quantity	Price	Lead time	Buy this product
SA100	50 ug	$22	in stock (MOQ 10 pcs.)
1A100	1 mg	$324	in stock

Found better price? Let us know and we will propose the way forward!

DAF-FM diacetate (DAF-FM DA) is a cell-permeant, fluorescent probe for detecting and quantifying low concentrations of nitric oxide (NO). DAF-FM DA passively diffuses across cellular membranes and, once inside cells, is deacetylated by intracellular esterases and converted to cell-impermeant form — DAF-FM.

The fluorescence quantum yield of DAF-FM is ~0.005, but it increases about 160-fold to ~0.81 after reacting with NO and forming a fluorescent benzotriazole (excitation/emission maxima at 495/515 nm) .

The NO detection limit of DAF-FM (~3 nM) is more sensitive than that of DAF-2 (~5 nM). The fluorescence of the NO adduct of DAF-FM is independent of pH above pH 5.5. Moreover, the NO adduct of DAF-FM demonstrates a significantly enhanced photostability compared to that of DAF-2, ensuring reliable results and additional time for imaging.

DAF-FM DA should be dissolved in DMSO and then used to prepare a working solution. Buffers containing bovine serum albumin (BSA) or phenol red can affect the fluorescence and should be used cautiously.

Download in
ChemDraw format

General properties

Appearance:	beige solid
Molecular weight:	496.42
CAS number:	254109-22-3
Molecular formula:	C₂₅H₁₈F₂N₂O₇
Solubility:	DMSO
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.